枸橼酸铁铵和脂多糖对星形胶质细胞LCN2表达的影响及机制  

EFFECT OF FERRIC AMMONIUM CITRATE AND LIPOPOLYSACCHARIDE ON THE EXPRESSION OF LIPOCALIN-2 IN PRIMARY CULTURED ASTROCYTES AND ITS MECHANISM

作　　者：唐硕 徐华敏[1] 谢俊霞[1] TANG Shuo;XU Huamin;XIE Junxia(Department of Physiology and Pathophysiology,School of Basic Medicine,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学基础医学院生理学与病理生理学系,山东青岛266071

出　　处：《青岛大学学报（医学版）》2021年第5期633-636,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金面上项目(31771124)。

摘　　要：目的探讨枸橼酸铁铵(FAC)和脂多糖(LPS)对原代培养的星形胶质细胞脂质运载蛋白-2(LCN2)表达的影响及可能的机制。方法实验分为对照组、FAC组、LPS组、FAC+LPS组、MG132+LPS组,对照组用细胞培养液处理,FAC组和LPS组分别用FAC和LPS处理24 h,FAC+LPS组先用FAC预处理4 h后再用LPS处理24 h,MG132+LPS组先用MG132预处理4 h后再用LPS处理24 h。应用蛋白质免疫印迹(Western blot)方法检测细胞内核因子κB(NF-κB)和LCN2的表达。结果与对照组相比,单独FAC处理不影响细胞内磷酸化的核因子κB(P-NF-κB)及LCN2的蛋白表达水平(F=11.76、68.18,q=0.469、0.655,P>0.05),LPS处理能够上调P-NF-κB及LCN2蛋白的表达(q=5.859、16.170,P<0.01);与LPS组相比,FAC预处理对LPS诱导的P-NF-κB及LCN2蛋白表达上调没有影响(q=1.516、1.151,P>0.05),而MG132预处理则能够显著抑制LPS诱导的P-NF-κB及LCN2蛋白表达上调(q=4.939、15.710,P<0.05)。结论细胞内高铁对LPS诱导的P-NF-κB和LCN2蛋白表达上调无明显影响,MG132能够下调LPS诱导的P-NF-κB和LCN2蛋白表达上调,蛋白酶体、NF-κB通路可能参与了LPS诱导的LCN2表达上调的抑制作用。Objective To investigate the effect of ferric ammonium citrate(FAC) and lipopolysaccharide(LPS) on the expression of lipocalin-2(LCN2) in primary cultured astrocytes and its possible mechanism. Methods Astrocytes were divided into control group, FAC group, LPS group, FAC+LPS group, and MG132+LPS group. The astrocytes in the control group were treated with cell culture media, those in the FAC group and the LPS group were treated with FAC or LPS for 24 h, those in the FAC+LPS group were given FAC pretreatment for 4 h followed by LPS treatment for 24 h, and those in the MG132+LPS group were given MG132 pretreatment for 4 h followed by LPS treatment for 24 h. Western blot was used to measure the protein expression of phosphorylated nuclear factor-kappa B(P-NF-κB) and LCN2 in astrocytes. Results Compared with the control group, FAC treatment alone did not affect the protein expression levels of P-NF-κB and LCN2 in astrocytes(F=11.76,68.18;q=0.469,0.655;P>0.05), and LPS treatment significantly upregulated the protein expression of P-NF-κB and LCN2(q=5.859,16.170;P<0.01). Compared with the LPS group, FAC pretreatment had no significant effect on the upregulated protein expression of P-NF-κB and LCN2 induced by LPS(q=1.516,1.151;P>0.05), and MG132 pretreatment significantly inhibited the upregulated protein expression of P-NF-κB and LCN2 induced by LPS(q=4.939,15.710;P<0.05). Conclusion High iron state in astrocytes has no significant effect on the upregulated protein expression of P-NF-κB and LCN2 induced by LPS, and MG132 can downregulate the upregulated protein expression of P-NF-κB and LCN2 induced by LPS. Proteasome and the NF-κB pathway may be involved in inhibition of the upregulated protein expression of LCN2 induced by PLS.

关 键 词：星形细胞 NF-κB 脂笼蛋白质类 铁 脂多糖类 

分 类 号：R338.2[医药卫生—人体生理学]