牵张应力环境中补肾活血汤干预成骨细胞增殖分化效果及机制研究  

Influence and mechanism of Bushen Huoxue decoction on proliferation of osteoblasts under stretch-stress environment

作　　者：汤小康[1] 唐成剑[1] 周长征[1] 卢敏[1] 李敏 应航 TANG Xiao-kang;TANG Cheng-jian;ZHOU Chang-zheng;LU Min;LI Min;YING Hang(First Hospital of Hunan University of Chinese Medicine,Changsha,Hunan,410007 China;School of Medical Technology,Zhejiang University of Chinese Medicine,Hangzhou,Zhejiang 310053 China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]浙江中医药大学医学技术学院,浙江杭州310053

出　　处：《时珍国医国药》2021年第7期1599-1602,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81574004,81874476);浙江省自然科学基金(Y2110928);湖南省卫生健康委科研课题(20201807,202104071103);湖南省中医药管理局科研计划项目(2021251);湖南中医药大学校级科研基金项目(ZYYDX201728)。

摘　　要：目的观察牵张应力环境中补肾活血汤对体外培养SD大鼠成骨细胞增殖分化效果,评估NO和PGE2在该过程中变化情况。方法制备补肾活血汤含药血清与空白对照血清,将其分别作用于SD大鼠成骨细胞。采用频率为0.5hz、形变量为12%的牵张应力,分别对含药血清组和空白血清对照组的成骨细胞进行力学加载。分别于加载时间的12 h和24 h,运用碱性磷酸酶活性试剂盒测定各组细胞ALP活性,运用MTT法检测各组细胞的增殖活性。力学加载时间的24 h后,通过硝酸还原酶法和ELISA法对各组细胞NO和PGE2表达量进行检测。运用统计学软件对相关结果进行数据分析。结果牵张应力环境影响SD大鼠成骨细胞24 h,能促进其增殖分化;牵张应力环境中补肾活血汤对体外培养SD大鼠成骨细胞增殖有促进作用;牵张应力环境中成骨细胞NO表达呈现抑制,牵张应力环境中补肾活血汤对体外培养SD大鼠成骨细胞NO表达增强;PGE2的表达均未发现明显变化。结论牵张应力环境中补肾活血汤能促进SD大鼠成骨细胞增殖与分化,这种作用的实现与其调控NO表达可能存在联系。Objective To observe the effect of Bushen Huoxue decoction on the proliferation and differentiation of SD rat osteoblasts in vitro, and to evaluate the changes of NO and PGE2 in the process. Methods The serum containing Bushen Huoxue decoction and blank control serum were prepared and treated on SD rat osteoblasts respectively. The osteoblasts in the drug containing serum group and blank serum control group were loaded with tensile stress of 0.5 Hz and 12% deformation respectively. ALP activity was measured by alkaline phosphatase activity kit and proliferation activity was detected by MTT method at 12 h and 24 h after loading. After 24 hours of mechanical loading, the expression of NO and PGE2 was detected by nitrate reductase and ELISA. Statistical software was used to analyze the related results. Results Stretch stress environment stimulate 24 h can affect the proliferation and differentiation of SD rat osteoblasts. In the stretch stress environment, Bushen Huoxue decoction can promote the proliferation of SD rat osteoblasts in vitro;the expression of NO in osteoblasts was inhibited in the stretch stress environment, the expression of NO in osteoblasts was increased by Bushen Huoxue decoction in the stretch stress environment. But the expression of PGE22 was not significantly changed. Conclusion Bushen Huoxue decoction can promote the proliferation and differentiation of SD rat osteoblasts in tension stress environment, which may be related to the regulation of NO expression.

关 键 词：牵张应力 成骨细胞 补肾活血汤 一氧化氮 前列腺素E 

分 类 号：R285.5[医药卫生—中药学]