^(137)Cs-γ辐照对实验红鲫的组织病理学影响  

Histopathological Effects of ^(137)Cs-γExternal Irradiation on Laboratory Red Crucian Carps

作　　者：陶宏婷 廖小立 彭赞平 李登科 陈科洁 吴端生[5] TAO Hongting;LIAO Xiaoli;PENG Zanping;LI Dengke;CHEN Kejie;WU Duansheng(Yongzhou Vocational Technical College,Yongzhou 425100,China;HuNan Institute of Traffic Engineering,Hengyang 421001,China;Yueyang Maternal and Child Health-care Hospital,Yueyang 414000,China;Hengyang Medical College,University of South China,Hengyang 421001,China;Department of Laboratory Animal Science,University of South China,Hengyang 421001,China)

机构地区：[1]永州职业技术学院,永州425100 [2]湖南交通工程学院,衡阳421001 [3]岳阳市妇幼保健院,岳阳414000 [4]南华大学衡阳医学院,衡阳421001 [5]南华大学实验动物学部,衡阳421001

出　　处：《实验动物科学》2021年第4期11-15,共5页Laboratory Animal Science

基　　金：“十二五”国家科技支撑计划项目(No.2015BAI09B05);湖南省重点研发计划项目(No.CX2017B542);湖南省教育厅科学研究项目(No.18C1408)。

摘　　要：目的探讨^(137)Cs-γ辐照对实验红鲫的组织病理学影响。方法用HXFS-IA生物辐照仪对实验红鲫进行^(137)Cs-γ一次性辐照,剂量分别为1.94 Gy、3.88 Gy、7.76 Gy和15.53 Gy。经^(137)Cs-γ辐照后的第14天,解剖实验红鲫取心脏、肝脏、肾脏、脾脏等组织,按常规方法进行石蜡切片及HE染色。结果实验红鲫经1.94~15.53 Gy^(137)Cs-γ辐照后,心肌纤维排列紊乱、结构不再完整;部分肝细胞出现水肿,继而出现坏死;脾脏中淋巴细胞逐渐减少,网状细胞和纤维组织增生;肾小管的上皮细胞水肿继而出现细胞坏死,即心脏、肝脏、脾脏与肾脏会随着核剂量的增加,其损伤程度加剧。结论在1.94~15.53 Gy剂量范围内,实验红鲫经^(137)Cs-γ一次性辐照后,心脏、肝脏、肾脏及脾脏均有组织病理损伤。随着辐照剂量的加大,组织损伤的程度增高。Objective To investigate the histopathological effects of^(137)Cs-γexternal irradiation on laboratory red crucian carps.Method The laboratory red crucian carps were subjected to^(137)Cs-γone-time external irradiation in HXFS-IA biological irradiator,with the irradiation doses 1.94 Gy,3.88 Gy,7.76 Gy and 15.53 Gy.On the 14 th day after irradiation,these carps were dissected and the tissues of heart,liver,kidney and spleen were taken out.Paraffin sections of the heart,liver,kidney and spleen were stained by HE using routine method.Result The red crucian carps showed some histopathological changes after irradiation from 1.94 to 15.53 Gy^(137)Cs-γ.The arrangement of myocardial fibers is disordered,and the structure of myocardial fibers is uncompleted.Some hepatocytes appeared edema,and then necrosis.In the spleen,lymphocytes gradually decreased and reticular cells and fibrous tissue proliferated.The epithelial cells of the renal tubules were edema,then appared cell necrosis.The heart,liver,spleen and kidneys were damaged with the increase of nuclear dose.Conclusion In the irradiation doses from 1.94 Gy to 15.53 Gy,the laboratory red crucian carps were exposed to^(137)Cs-γfor one-time external irradiation,and the heart,liver,kidney and spleen got histopathological damaged.The degree of tissue damages with the increase of radiation dose was exacerbation.

关 键 词：^(137)Cs-γ 实验红鲫 器官组织 组织病理学 

分 类 号：S852.31[农业科学—基础兽医学]