MiR-497-5p对紫杉醇耐药乳腺癌细胞增殖和凋亡的影响  

Effects of miR-497-5p on proliferation and apoptosis of paclitaxel resistant breast cancer cells

作　　者：马友龙[1] 延靖蕾 祁海艳[3] 马立辉[1] MA You-long;YAN Jing-lei;QI Hai-yan;MA Li-hui(Department of Breast Surgery,Affiliated Hospital of Chengde Medical University,Chengde 067000,Hebei Province,China;Department of Orthopedics,Chengde Central Hospital,Chengde 067000,Hebei Province,China;Department of Surgery in South Campus,Affiliated Hospital of Chengde Medical University,Chengde 067000,Hebei Province,China)

机构地区：[1]承德医学院附属医院乳腺外科,河北承德067000 [2]承德市中心医院骨科,河北承德067000 [3]承德医学院附属医院南院手术室,河北承德067000

出　　处：《中国临床药理学杂志》2021年第20期2763-2765,2769,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探讨miR-497-5p对乳腺癌紫杉醇耐药细胞的影响,并探讨其作用机制。方法取乳腺癌紫杉醇耐药细胞系MCF-7/紫杉醇。将MCF-7/紫杉醇细胞,分别转染miR-497-5p模拟物(miR-497-5p mimics组)、miR-497-5p模拟物阴性对照(miR-NC组)、miR-497-5p抑制剂(miR-497-5p inhibitor组)及miR-497-5p抑制剂阴性对照(NC组)。用实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测miR-497-5p表达,用噻唑蓝(MTT)法检测细胞增殖情况,用流式细胞术检测细胞凋亡情况,用蛋白质印迹(Western blot)法检测程序性死亡配体1(PD-L1)蛋白的表达。结果miR-497-5p在MCF-7/紫杉醇细胞中表达水平0.44±0.02,显著低于MCF-7细胞的0.73±0.02,差异有统计学意义(P<0.05)。转染48 h后miR-NC组、miR-497-5p mimics组、NC组及miR-497-5p inhibitors组细胞增殖抑制率分别为(19.67±2.08)%,(46.33±5.86)%,(17.33±1.53)%,(9.33±3.51)%;细胞凋亡率分别为(4.18±0.07)%,(16.37±1.10)%,(5.56±0.41)%和(2.44±0.13)%,以上指标,miR-NC组与miR-497-5p mimics组相比,NC组与miR-497-5p inhibitors组相比,差异均有统计学意义(均P<0.05)。miR-497-5p mimics组中PD-L1蛋白表达显著较miR-NC组低;miR-497-5p in-hibitor组中PD-L1蛋白表达显著较NC组高,差异有统计学意义(P<0.05)。结论miR-497-5p通过调控PD-L1表达抑制乳腺癌紫杉醇耐药细胞增殖并促进细胞凋亡。Objective To investigate the role of miR-497-5p in the proliferation and apoptosis of breast cancer paclitaxel(PTX)-resistant cells,and to explore its mechanism.Methods MCF-7/PTX cells were transfected with miR-497-5p mimics(miR-497-5p mimics group),miR-497-5p mimics negative control(miR-NC group),miR-497-5p inhibitor(miR-497-5p inhibitor group)and miR-497-5p inhibitor negative control(NC group).The expression of miR-497-5p was detected by real-time quantitative polymerase chain reaction(qRT-PCR).Cell proliferation was detected by MTT;cell apoptosis was detected by flow cytometry.Western blot was used to detect the expression of programmed death-ligand 1(PD-L1)protein.Results The expression level of miR-497-5p in MCF-7/PTX cells was 0.44±0.02,significantly lower than that in MCF-7 cells,which was 0.73±0.02,the difference was statistical significance(P<0.05).After transfected for 48 h,the inhibition rate of cell proliferation in miR-NC group,miR-497-5p mimics group,NC group and miR-497-5p inhibitor group were(19.67±2.08)%,(46.33±5.86)%,(17.33±1.53)%,(9.33±3.51)%,respectively;the apoptosis rates were(4.18±0.07)%,(16.37±1.10)%,(5.56±0.41)%and(2.44±0.13)%,respectively.Comparison between miR-NC and miR-497-5p mimics groups,between NC group and miR-497-5p inhibitor group,the differences of the above factors were significant(all P<0.05).The expression of PD-L1 in miR-497-5p mimics group was lower than that in miR-NC group,and the expression of PD-L1 was higher in miR-497-5p inhibitor group than that in NC group,the differences were statistically significant(P<0.05).Conclusion miR-497-5p inhibit the proliferation and promoted apoptosis of breast cancer cell resistance to paclitaxel chemotherapy by regulating PD-L1 expression.

关 键 词：化疗耐药 乳腺肿瘤 程序性死亡配体1 紫杉醇 

分 类 号：R979.1[医药卫生—药品]