微小染色体缺陷维持蛋白3对黑色素瘤细胞迁移和侵袭的影响  

Effect of minichromosome maintenance deficient 3(MCm3)on migration and invasion of melanoma cells

作　　者：高琼[1] 刘昱昕 李伟娥 李可心 王立鹏[1] GAO Qiong;LIU Yu-xin;LI Wei-e;LI Ke-xin;WANG Li-peng(Department of Dermatology,General Hospital of Ningxia Medical University,Yinchuan 750000,Gansu Province,China;Department of Dermatology,Yinchuan Maternal and Child Health Hospital,Yinchuan 750001,Gansu Province,China)

机构地区：[1]宁夏医科大学总医院皮肤科,甘肃银川750000 [2]银川市妇幼保健院皮肤科,甘肃银川750001

出　　处：《中国临床药理学杂志》2021年第20期2798-2801,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探究微小染色体缺陷维持蛋白3(MCm3)通过Wnt/β-catenin信号通路对黑色素瘤细胞迁移和侵袭的影响。方法将人恶性黑色素瘤细胞A375分为对照组(空白对照),si-NC组(转染si-NC组)、si-MCm3组(转染si-MCm3组)。以噻唑蓝(MTT)法检测各组细胞活力,以划痕实验和Transwell法检测各组细胞的迁移和侵袭能力,以蛋白质印迹(Western blot)法检测Wnt3a蛋白(Wnt3a)、β-连环蛋白(β-catenin)、细胞周期蛋白D1(Cyclin D1)表达水平。结果对照组、si-NC组和si-MCm3组72 h的细胞存活率分别为(249.31±18.66)%,(253.26±12.63)%,(168.71±16.01)%,侵袭细胞数分别为(375.55±11.83),(366.94±20.04),(148.53±11.75)个,划痕愈合率分别为(53.64±5.71)%,(51.37±4.33)%和(22.37±2.07)%,si-MCm3组与对照组和si-NC组相比,差异均有统计学意义(均P<0.05)。与对照组和si-NC相比,si-MCm3组细胞Wnt3a、β-catenin、Cyclin D1均显著降低(均P<0.01)。结论敲低MCm3可抑制黑色素瘤细胞的迁移和侵袭,其机制可能与Wnt/β-catenin信号通路相关.Objective To investigate the effect of minichromosome maintenance deficient 3(MCM3) on the migration and invasion of melanoma cells through Wnt/β-catenin signaling pathway. Methods A375 cells were divided into control group(blank control), Si-NC group(transfected with si-NC) and si-MCM3 group(transfected with si-MCM3). MTT assay was used to detect cell viability, scratch test and Transwell assay were used to detect cell migration and invasion, and Western blot was used to detect the expression of Wnt3 a protein(Wnt3 a), β-catenin and Cyclin D1. Results The cell survival rate in control group, si-NC group and si-MCM3 group at 72 h were(249.31±18.66) %,(253.26±12.63) %,(168.71±16.01) %, and the cell invasion number were(375.55±11.83),(366.94±20.04),(148.53±11.75), the scratch healing rates were(53.64±5.71) %,(51.37±4.33) % and(22.37±2.07) %, respectively. Compared with control group and si-NC group, the differences of above indexes in si-MCM3 group were statistically significant( all P < 0. 05). The levels of Wnt3 a,β-catenin and Cyclin D1 were significantly decreased in si-MCM3 group compared with control group and si-NC group( all P < 0. 01). Conclusion MCM3 plays an important role in promoting the migration and invasion of melanoma cells,and its mechanism may be related to the activation of Wnt/β-catenin signaling pathway.

关 键 词：微小染色体缺陷维持蛋白3(MCm3) Wnt/β-连环蛋白信号通路(Wnt/β-catenin) 黑色素瘤 迁移 侵袭 

分 类 号：R97[医药卫生—药品]