沙枣籽中微晶纤维素的分离和特征分析  

Isolation and Characterization of Microcrystalline Cellulose from Elaeagnus Angustifolia Seed

作　　者：付瑞敏 王丁 于烽 陈五岭 FU Ruimin;WANG Ding;YU Feng;CHEN Wuling(College of Health Management,Henan Finance University,Zhengzhou 450046,China;College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi’an 710069,China)

机构地区：[1]河南财政金融学院健康管理学院,河南郑州450046 [2]陕西师范大学食品工程与营养科学学院,陕西西安710069

出　　处：《食品科技》2021年第9期260-265,共6页Food Science and Technology

基　　金：国家留学基金委地方合作项目(10006);河南省科技攻关项目(182102110002,182102110995);河南省高等学校青年骨干教师资助计划项目(2020GGJS265);河南省高等学校重点科研项目(21B180002)。

摘　　要：目的:探讨从沙枣籽中提取微晶纤维素的方法及所得微晶纤维素的成分表征。方法:采用"三步法"(脱蜡、脱木质化和酸水解)从沙枣籽中分离出微晶纤维素,而后使用傅立叶变换红外光谱、扫描电镜、透射电镜、热重分析等方法研究所得样品的形态结构表征及热稳定性。结果:红外光谱分析结果显示,通过对沙枣籽进行脱木质化及漂白处理,原来包裹在纤维素基质表面的木质素和半纤维素等无定形成分已被成功去除;扫描电镜结果分析表明,所获得的微晶纤维素具有不规则的、团聚的细长或半球形结构,该结构同作为药用辅材及食品加工的商售微晶纤维素类似;透射电镜的结果展示了微晶纤维素中的晶格形态及半结晶性质;热重分析结果表明从沙枣籽中提取的微晶纤维素具有较强的热稳定性。结论:研究表明,沙枣籽作为微晶纤维素的低价提取来源,在制药及食品行业中具有广阔的应用前景。Objective:To explore the method of extracting microcrystalline cellulose(MC)from Elaeagnus angustifolia seeds and the characterization of MC composition.Methods:Microcrystalline cellulose was isolated from Elaeagnus angustifolia seeds by dewaxing,delignification and acid hydrolysis and studied for its morphological structure and thermal stability using Fourier transform infrared spectroscopy(FTIR),scanning electron microscopy(SEM),transmission electron microscopy(TEM)and thermogravimetric(TG)studies.Results:FTIR analysis of delignified Elaeagnus angustifolia seeds showed that amorphous components such as lignin and hemicellulose,which were previously wrapped around the surface of the cellulose matrix,had been successfully removed.Analysis of the SEM results showed that the obtained MC exhibited agglomerated and irregular elongated or hemispherical morphological features,which were similar in shape to that of commercially available MC used as a pharmaceutical excipient.The results of transmission electron microscopy analysis confirm the semi-crystalline nature of MC.Thermal analysis showed enhanced thermal stability of MC.Conclusion:This study shows that Elaeagnus angustifolia seeds can be used as a low-cost source of MC for the broad application prospect in pharmaceutical and food industry.

关 键 词：沙枣籽 纤维素 微晶纤维素 成分表征 热重分析 

分 类 号：R284.2[医药卫生—中药学]