‘哈伯’南天竹组织培养和快速繁殖  

Nandina domestica‘Harbour Dwarf’:Tissue Culture and Rapid Propagation

作　　者：张俊林 樊靖 胡玉春 杨浩 胡绍庆[2] 沈柏春 Zhang Junlin;Fan Jing;Hu Yuchun;Yang Hao;Hu Shaoqing;Shen Bochun(Hangzhou Landscaping Incorporated,Hangzhou 31000,Zhejiang,China;Building Engineering College,Zhejiang Sci-Tech University,Hangzhou 310018,Zhejiang,China)

机构地区：[1]杭州市园林绿化股份有限公司,杭州310005 [2]浙江理工大学建筑工程学院,杭州310018

出　　处：《农学学报》2021年第10期63-68,89,共7页Journal of Agriculture

基　　金：浙江省农业新品种选育重大科技专项“特色优势木本花卉资源保育与种质创新”(2016C02056-12)。

摘　　要：为建立‘哈伯’南天竹组织培养和种苗繁育技术体系,以半木质化带芽茎段为外植体材料开展植株再生研究。通过观察对比试验法、L9(34)正交试验设计完全随机法、极差分析、显著性检验、LSD多重比较,探讨了‘哈伯’南天竹组培的最适培养基配方。试验结果表明:最佳诱导培养基为MS+6-BA 2.0 mg/L+IBA 0.1 mg/L+蔗糖30 g/L,诱导萌动率71.77%,成活率85.51%;最佳增殖培养基为WPM+6-BA 1.5 mg/L+IBA 0.01 mg/L+蔗糖30 g/L,增殖系数6.3;最佳生根培养基为1/2 MS+IBA 0.5 mg/L+NAA 1.0 mg/L+蔗糖20 g/L+AC 0.2 g/L,生根率97.63%;试管苗移入泥炭土:珍珠岩=3:2(V/V)混合基质中,移栽成活率96.67%。该试验建立了高效稳定的组培快繁技术体系,得到的组培苗后代能够稳定的保持母本优良性状,为工厂化育苗提供了技术支撑。The aim of this study is to establish a system of tissue culture and seedling production for Nandina domestica‘Harbour Dwarf’.The stem segments of semi-lignified band buds were used as explants to conduct regeneration study.The comparative experiments,completely randomized orthogonal experiment design of L_(9)(3^(4)),range analysis,significance test and LSD multiply comparative analysis were used to discuss the optimal culture medium.The results showed that the highest frequency of shoot induction(71.77%)and survival rate(85.51%)were achieved on MS+6-BA 2.0 mg/L+IBA 0.1 mg/L+sucrose 30 g/L.The highest proliferation coefficient(6.3)was obtained on WPM+6-BA 1.5 mg/L+IBA 0.01 mg/L+sucrose 30 g/L.Microshoots exhibited best rooting response on 1/2 MS+IBA 0.5 mg/L+NAA 1.0 mg/L+sucrose 20 g/L+AC 0.2 g/L by producing the highest frequency of root formation(97.63%).Rooted plantlets were transferred to artificial mixture medium(peat:perlite=3:2.V/V)under the green house condition with the survival rate up to 96.67%.An efficient propagation system of Nandina domestica‘Harbour Dwarf’was established,and the plantlets could steadily inherit the character of parents.The present system provides technical support for large scale plant production.

关 键 词：‘哈伯’南天竹 组织培养 种苗繁育 再生 增殖 

分 类 号：S688.4[农业科学—观赏园艺]