HNRNPU在重度少、弱精子症的表达及其对卵泡浆内单精子显微注射治疗临床结局的影响  

Heterogeneous Nuclear Ribonucleoprotein U expression in patients with severe oligoasthenospermia and its effect on clinical outcome of intracytoplasmic sperm injection

作　　者：梁嘉颖[1] 曾伟宏[2] 张杰[1] 赖有行[3] 黄志承[1] 郑毅春[1] LIANG Jiaying;ZENG Weihong;ZHANG Jie;LAI Youxing;HUANG Zhicheng;ZHENG Yichun(Department of Reproductive Health and Fertility,Guang-dong Women and Children Hospital,Guangzhou 510010,China)

机构地区：[1]广东省妇幼保健院生殖健康与不孕症科,广州510010 [2]广东省妇幼保健院儿童遗传代谢与内分泌科,广州510010 [3]广东省妇幼保健院检验科,广州510010

出　　处：《实用医学杂志》2021年第19期2468-2472,共5页The Journal of Practical Medicine

基　　金：广州市科技计划项目(编号:201707010375,202102080021);广东省医学科学技术研究基金项目(编号:A2020061)。

摘　　要：目的探讨严重少、弱精子症患者精液精子中HNRNPU的表达及其与精子质量参数的相关性,探讨其对卵泡浆内单精子显微注射(ICSI)治疗临床结局的影响。方法选取严重少、弱精子症需行ICSI治疗患者和正常生育男性各500例,收集精液,采用RT-PCR检测精子HNRNPU mRNA;计算机辅助精液分析系统(CASA)分析精液常规参数;Diff-Quik染色法分析精子形态;采用钙离子载体A23187诱导精子发生顶体反应(ARIC);SCD法检测精子DNA碎片化指数(DFI);苯胺蓝染色法检测精子核成熟度;记录ICSI组的治疗结局。结果ICSI组HNRNPU mRNA相对量显著高于正常组精子[(0.38±0.04)vs.(0.14±0.03),P<0.05],ICSI组正常形态精子百分率、ARIC%和核成熟度均低于正常组,差异均有统计学意义(P<0.001);HNRNPU mRNA与前向运动精子总数、正常形态精子百分率、ARIC%和核成熟度呈负相关,与DFI呈正相关(P<0.001);选择研究时间内,女方采用长效长方案促排卵,并行新鲜胚胎移植的104个ICSI治疗周期,男方精子HNRNPU基因mRNA与前向运动精子总数和正常受精(2PN)率呈负相关(P<0.05),但与卵裂率、优胚率和临床妊娠率无显著相关性。结论精子HNRNPU mRNA表达上调可能是严重少、弱精子症发病的原因之一;对ICSI受精率有一定的预测作用。Objective(HNRNPU)expression and sperm quality parameters in patients with severe and weak sperm disease,and to explore its effect on the clinical outcome of intracytoplasmic sperm injection(ICSI).Methods specimens from men with severe oligoasthenozoospermia requiring ICSI treatment(ICSI group)and 500 from normal men(control group)were collected at our clinic.The sperm HNRNPU mRNA,volume,concentration,morphology,total progressive motile sperm count,Ca2+ionophore(A23187)-induced arcrosome reaction(ARIC)rate,sperm DNA fragmentation index(DFI)and sperm nucleation were detected and outcomes in the ICSI group were recorded.Results than the control group(0.14±0.03)(P<0.05),and the normal morphology sperm,ARIC%and maturity in ICSI group were significant lower than those in the control group(P<0.001).HNRNPU mRNA was negatively related with total progressive motitle sperm count,normal morphology sperm,ARIC%and maturity,but positively with DFI(P<0.001).HNRNPU mRNA was negatively related with normal fertilization(2 PN)rate(P<0.05),but not with cleavage rate or clinical pregnancy rate of ICSI.Conclusion may be one of the reasons for the onset of severe oligoasthenozoospermia.It can predict the fertility rate of ICSI to a certain extent.

关 键 词：HNRNPU 少精子症 弱精子症 卵泡浆内单精子注射 

分 类 号：R698.2[医药卫生—泌尿科学]