快速冻融睾丸精子在无精子症患者中应用的潜在影响  

Potential Impact of Rapid Freezing and Thawing of Testicular Sperm in Patients with Azoospermia

作　　者：董竞 赵激浪 欧阳杰 张伟[1] 马猛 王田田 罗沛琦 陆诗佳 彭惠 李斌[1] Dong Jing;Zhao Jilang;Ouyang Jie(Department of Assisted Reproduction,Ninth People′s Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200011,China)

机构地区：[1]上海交通大学医学院附属第九人民医院辅助生殖科,200011

出　　处：《医学研究杂志》2021年第10期40-45,共6页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81971448)。

摘　　要：目的通过比较快速冻融睾丸精子和新鲜睾丸精子行胞质内单精子注射(ICSI)后的多种临床指标,探讨睾丸精子快速冻融对无精子症患者治疗结局的影响。方法用倾向性评分匹配方法对患者基本资料进行匹配后,共回顾性分析了272个ICSI周期。通过比较两组中患者的受精率、优胚率、卵子利用率、临床妊娠率和活产率等多种指标来探讨睾丸精子的快速冻融对患者临床结局的影响。结果两组的优胚率、卵子利用率、临床妊娠率、活产率等比较,差异无统计学意义(P>0.05),而研究组患者的受精率、正常受精率显著低于对照组(P<0.05)。结论睾丸精子快速冻融未对无精子症患者的临床结局带来不利影响,对其进行提前快速冻存有利于患者临床治疗安排。Objective To explore the effect of rapid freezing and thawing testicular sperm on the treatment outcome of azoospermia patients by comparing various clinical indicators of rapid freezing and thawing testicular sperm and fresh testicular sperm after intracytoplasmic sperm injection(ICSI).Methods After matching the basic data of patients with the propensity score matching method,a total of 272 ICSI cycles were retrospectively analyzed.The study compares the fertilization rate,high-quality embryo rate,oocytes utilization rate,clinical pregnancy rate and live birth rate of patients in the two groups to explore influence of rapid freezing and thawing of testicular sperm on the clinical outcome of patients.Results There was no statistical difference between the two groups in the rate of high-quality embryo rate,oocytes utilization rate,clinical pregnancy rate,implantation rate and live birth rate(P>0.05),while the fertilization rate and normal fertilization rate of patients in the frozen-thawed sperm group were significantly lower than those in the control group(P<0.05).Conclusion The rapid freezing and thawing of testicular sperm did not adversely affect the clinical outcome of patients with azoospermia,and rapid freezing in advance is beneficial to the clinical treatment of patients.

关 键 词：冻融 睾丸精子 ICSI 

分 类 号：R711.6[医药卫生—妇产科学]