饮水砷暴露对SD大鼠肝脏损伤的形态学研究  

Morphological Study of Liver Injury in SD Rats Induced by Arsenic Exposure in Drinking Water

作　　者：丁关鑫 黄佳[2] 夏荣香[2] 林勤[2] 马艳[1] 吴顺华[1] 张玲[2] Ding Guanxin;Huang Jia;Xia Rongxiang(School of Public Health,Xinjiang Medical University,Xinjiang 830011,China)

机构地区：[1]新疆医科大学公共卫生学院,乌鲁木齐830011 [2]新疆维吾尔自治区疾病预防控制中心,乌鲁木齐830002

出　　处：《医学研究杂志》2021年第10期45-48,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81560520)。

摘　　要：目的研究不同浓度亚砷酸钠染毒对大鼠肝脏超微结构变化的影响。方法将56只健康成年SD大鼠随机分为对照组、低剂量组、中剂量组、高剂量组。记录尿砷含量以及观察肝脏病理形态学改变的情况。结果低、中、高剂量组的尿砷含量均大于对照组,经检验差异有统计学意义(Z=48.50,P<0.01),且随着亚砷酸钠剂量的增大,尿砷的含量也随着升高。与对照组比较,中、高剂量组的肝脏重量下降明显,经检验,差异有统计学意义(F=3.246,P<0.05)。电镜下观察,低剂量组肝细胞细胞核异形,中、高剂量组出现细胞核畸形,肝细胞灶性坏死。结论亚慢性饮水砷暴露会造成肝脏超微结构变化,且随着亚砷酸钠剂量的增加,肝脏的病理变化也随之加重。同时,SD大鼠尿砷含量增高,肝脏重量下降。Objective To study the effects of different doses of sodium arsenite on the ultrastructure of rat liver.Methods Fifty-six healthy adult SD rats were randomly divided into 4 groups:control group,low-dose group,medium-dose group,high-dose group.Urine arsenic content was recorded and liver pathomorphology was observed.Results The urinary arsenic content of the low-dose group,medium-dose goup,high-dose group were higher than that of the control group,the difference was statistically significant(Z=48.50,P<0.01),and the urinary arsenic content increased with the increase of the exposure dose.Compared with control group,liver weight of the medium-dose group and high-dose group decreased significantly,and the difference was statistically significant(F=3.246,P<0.05).Under electron microscope,the liver nucleus was abnormal in the low-dose group,and nuclear abnormality and focal necrosis of liver cells were observed in the medium-dose group and high-dose group.Conclusion Subchronic arsenic exposure in drinking water can cause ultrastructural changes in the liver,and with the increase of sodium arsenite dose,the pathological changes in the liver are also aggravated.Meanwhile,arsenic content in the liver of SD rats increases,and the weight of the liver decreases.

关 键 词：饮水砷暴露 肝脏 病理形态学 

分 类 号：R595.2[医药卫生—内科学]