不同碘摄入水平对子代大鼠学习记忆及运动能力的影响  

Effects of Different Iodine Intake Levels on Learning and Memory and Motor Ability in Offspring Rats

作　　者：黄佳[1] 王琛琛[1] 祝宇铭[1] 郜振国[1] 樊玉娟 马品江[1] Huang Jia;Wang Chenchen;Zhu Yuming(Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region,Xinjiang 830002,China)

机构地区：[1]新疆维吾尔自治区疾病预防控制中心,乌鲁木齐830002

出　　处：《医学研究杂志》2021年第10期79-82,共4页Journal of Medical Research

基　　金：新疆维吾尔自治区卫生健康青年医学科技人才专项科研项目(WJWY-202016)。

摘　　要：目的探讨不同碘摄入水平对子代大鼠神经发育行为的影响。方法断乳2周的SPF级雌性Wistar大鼠150只,按体质量随机分成5组,分别是碘缺乏1组(severe iodine deficiency,SID)、碘缺乏2组(mild iodine deficiency,MID)、适碘对照组(normal iodine,NI)、碘过量1组(mild iodine excess,MIE)和碘过量2组(severe iodine excess,SIE),各组饮水碘酸钾浓度依次为0、50、183、2283和11617μg/L,干预3个月,对子代进行Morris水迷宫实验、平衡木实验、钢丝夹持实验、转棒实验。结果5个剂量组子代大鼠平衡木通过时间及在平衡木上发生打滑次数、在钢丝上的悬挂时间比较差异有统计学意义(F=2.640,χ^(2)=11.873,F=3.178,P<0.05),均表现出适碘对照组子代大鼠具有较强的运动能力;定位巡航实验发现,第2天和第3天各剂量组子代大鼠逃避潜伏期时间均比NI组长(P<0.05)。空间探索实验显示,SID组和SIE组较NI组有效区域运动时间有所延长(P<0.05),各组均比NI组穿过平台的次数少(P<0.05)。结论孕期不同程度的碘缺乏和碘过量均会损伤子代大鼠的学习记忆及运动能力。Objective To investigate the effects of different iodine intake levels on neurodevelopmental behavior in offspring rats.Methods A total of 150 SPF female Wistar rats(ablactation for 2 weeks)were randomly divided into 5 groups according to body weight,including iodine deficiency 1(severe iodine deficiency,SID),iodine deficiency 2(mild iodine deficiency,MID),normal iodine control group(normal iodine,NI),iodine excess 1(mild iodine excess,MIE)and iodine excess 2(severe iodine excess,SIE).The concentrations of potassium iodate in drinking water of each group were in 0,50,183,2283 and 11617μg/L.After 3 months of intervention,morris water maze test,balance beam test,steel wire gripping test and rotating bar test were performed on the offspring.Results There were statistically significant differences in the time of passing the balance beam,the times of slipping on the balance beam and the time of hanging on the steel wire(F=2.640,χ^(2)=11.873,F=3.178,P<0.05).All the results showed that the offspring rats in the appropriate iodine control group had strong motor ability.The location cruise experiment showed that the escape latency time of offspring rats in all dose groups on day 2 and day 3 was longer than that of control group(P<0.05).The space exploration experiment showed that the movement time of effective area in SID group and SIE group was longer than that in NI group(P<0.05).The number of crossing platform in each group was less than that in NI group(P<0.05).Conclusion Iodine deficiency and iodine excess during pregnancy can damage the learning and memory and motor ability of offspring rats.

关 键 词：碘 神经发育 学习记忆 行为学 运动能力 

分 类 号：R195[医药卫生—卫生统计学]