分子印迹的SiO_(2)荧光探针制备及对儿茶酚的探测  

Preparation for SiO_(2) Fluorescence Probe of Molecule Imprinting with NBD Fluorescence Label and Its Detection to Catechol

作　　者：孙文潭 程燕[2] 张颍 SUN Wen-tan;CHENG Yan;ZHANG Ying(Anhui Wanxin Environmental Technology Co.,Ltd.,Anhui Hefei 230000;Hefei Environmental Monitoring Center Station,Anhui Hefei 230000,China)

机构地区：[1]安徽皖欣环境科技有限公司,安徽合肥230000 [2]合肥市环境监测中心站,安徽合肥230000

出　　处：《广州化工》2021年第21期43-46,共4页GuangZhou Chemical Industry

摘　　要：以儿茶酚为目标分子,APTS为功能单体,TEOS为交联剂,NBD-APTS为荧光标记探针,运用纳米技术、表面功能化修饰和分子印迹技术在二氧化硅表面制备了儿茶酚分子印迹的NBD-NH_(2)-SiO_(2)荧光探针,洗脱了儿茶酚分子的SiO_(2)表面,拥有空间结构与儿茶酚分子相匹配的空穴,实现对儿茶酚选择性识别,识别位点处标记的荧光探针,基于光诱导电子转移的原理,通过荧光强度的增强,实现对目标分子敏感探测。Herein,an NBD-NH_(2)-SiO_(2) nanoparticle fluorescence probe with high-selective recognition and high-sensible detection to catechol was synthesized through the copolymerization of catechol as template molecule,APTS as functional monomer,TEOS as crossing linker and NBD as labeled fluorescence,using nano-sythesized technique,surface functionalization modification and molecular imprinting technique.The recognition sites with catechol molecules eluted on nanoparticles surface had cavities matching the spatial structure of catechol molecules,which can realize the selective recognition of catechol and the NBD was labeled as the fluorescence probe at the recognition sites.Based on the principle of photoinduced electron transfer,it can carry out the sensitive detection of target molecules through intensity change of the fluorescence probe in the recognition sites.

关 键 词：分子印迹 分子识别 荧光标记 光诱导电子转移 儿茶酚 

分 类 号：X703.1[环境科学与工程—环境工程]