基于响应面法优化无子刺梨总皂苷提取条件  

Optimization of Extraction Condition for Total Saponins in Rosa Sterilis by Response Surface Methodology

作　　者：李楚然 但汉龙[1] 刘云[1] 罗旭璐 李永和 李自元 赵平[1] LI Chu-ran;DAN Han-long;LIU Yun;LUO Xu-lu;LI Yong-he;LI Zi-yuan;ZHAO Ping(Key Laboratory of State Forestry and Grassland Administration on Highly-efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming 650224,China;Yunnan Forestry Technological College,Kunming 650224,China;Guizhou Lyuyinhe Agricultural Development Co.,Ltd.,Xingren 562306,China)

机构地区：[1]西南林业大学西南地区林业生物质资源高效利用国家林业和草原局重点实验室,昆明650224 [2]云南林业职业技术学院,昆明650224 [3]贵州绿荫河农业发展有限公司,贵州兴仁562306

出　　处：《包装工程》2021年第21期25-32,共8页Packaging Engineering

基　　金：推进黔西南试验区建设科技合作专项资金(黔西南科合2015-31);西南林业大学科技创新基金(C15081)。

摘　　要：目的优化超声辅助提取无子刺梨总皂苷的条件,以提高总皂苷的提取效率。方法通过单因素试验评估乙醇体积分数、超声时间、料液比和提取次数对总皂苷得率的影响,结合响应面法的中心组合实验设计,确定其最佳提取条件。结果在超声时间为40 min、乙醇体积分数为60%、料液比(g/mL)为1∶30、提取4次的条件下,总皂苷的得率为9.15%,与模型预测值9.18%较为接近,表明了响应面法优化无子刺梨总皂苷的超声辅助提取方法的可行性。结论此研究为无子刺梨资源在食品贮藏、医药等方面的进一步开发利用提供参考依据。The work aims to optimize the extraction condition for total saponins from Rosa sterilis by ultrasonic-assisted method,so as to improve the extraction efficiency.The effects of ethanol volume fraction,ultrasonic time,material-liquid ratio,and extraction number on the extraction yield of total saponins were investigated by single factor experiment method,and then the central composite design of response surface methodology was carried out to determine the optimum extraction conditions.The optimal extraction conditions were defined as ultrasonic time of 40 min,ethanol volume fraction of 60%,material-liquid ratio of 1∶30 g/mL,and extraction number of 4 times.Under these optimal conditions,the actual extraction yield of total saponins was 9.15%,which was almost equal to the predicted value of 9.18%.These results indicated that the response surface methodology was feasible to optimize the ultrasonic-assisted extraction conditions of total saponins from Rosa sterilis.The work provides reference for the exploitation and utilization of Rosa sterilis in food preservation and medicine.

关 键 词：无子刺梨 皂苷 超声辅助 提取 响应面 

分 类 号：S789[农业科学—林学]