阿托伐他汀对白蛋白诱导的HK-2细胞凋亡的影响及机制初探  

Effect of Atorvastatin on Albumin-Induced Apoptosis in HK-2 Cells and Its Mechanism of Action

作　　者：陈艳霞[1] 黄翀[1] 秦晓华[1] 房向东[1] 涂卫平[1] CHEN Yan-xia;HUANG Chong;QIN Xiao-hua;FANG Xiang-dong;TU Wei-ping(Department of Nephrology,the Second Affiliated Hospital of Nanchang University,Nanchang 330006,China)

机构地区：[1]南昌大学第二附属医院肾脏内科,南昌330006

出　　处：《南昌大学学报（医学版）》2021年第5期19-23,共5页Journal of Nanchang University：Medical Sciences

基　　金：江西省卫生计生委科技计划(20185218)。

摘　　要：目的探讨阿托伐他汀(atorvastatin,ATO)对白蛋白诱导的人肾小管上皮细胞(human renal tubular epithelial cells,HK-2)凋亡的影响,并对其可能作用机制进行初步探讨。方法体外培养HK-2细胞,将培养到85%~90%的HK-2细胞随机分为7组:空白对照组(A组)、白蛋白诱导组(B组:5 mg·mL^(-1)白蛋白)、ATO组(C组:10-8 mol·L^(-1)ATO)、低浓度ATO+白蛋白诱导组(D组:10^(-1)0 mol·L^(-1)ATO+5 mg·mL^(-1)白蛋白)、中浓度ATO+白蛋白诱导组(E组:10-9 mol·L^(-1)ATO+5 mg·mL^(-1)白蛋白)、高浓度ATO+白蛋白诱导组(F组:10-8 mol·L^(-1)ATO+5 mg·mL^(-1)白蛋白)、RhoA/ROCK信号通路阻断剂组(G组:10μmol·L^(-1)Y27632+5 mg·mL^(-1)白蛋白);予以各实验组细胞干预培养48 h后,流式细胞仪检测各组HK-2细胞的早期凋亡,采用RT-PCR检测各组HK-2细胞RhoAmRNA、ROCK1mRNA的表达。结果空白对照组与ATO组HK-2细胞的早期凋亡、RhoAmRNA、ROCK1mRNA的表达比较差异均无统计学意义(均P>0.05);不同浓度的ATO+白蛋白诱导组及RhoA/ROCK信号通路阻断剂组HK-2细胞较白蛋白诱导组早期凋亡降低(均P<0.05),且随着ATO浓度的升高,其抑制HK-2细胞早期凋亡的作用越明显;不同浓度的ATO+白蛋白诱导组HK-2细胞RhoAmRNA、ROCK1 mRNA的表达较白蛋白诱导组减少(均P<0.05)。结论ATO可抑制白蛋白诱导的HK-2细胞的早期凋亡,其作用机制可能与部分阻断RhoA/ROCK信号通路有关。Objective To investigate the effect of atorvastatin(ATO)on albumin-induced apoptosis in human renal tubular epithelial cells(HK-2),and to explore its possible mechanism of action.Methods HK-2 cells grown to 85%-90%confluence were randomly divided into 7 groups:blank control group(group A),5 mg·mL^(-1)albumin induction group(group B),10-8 mol·L^(-1)ATO treatment group(group C),10^(-1)0 mol·L^(-1)ATO+5 mg·mL^(-1)albumin group(group D),10-9 mol·L^(-1)ATO+5 mg·mL^(-1)albumin group(group E),10-8 mol·L^(-1)ATO+5 mg·mL^(-1)albumin group(group F),and(10μmol·L^(-1)Y27632+5 mg·mL^(-1)RhoA/ROCK signal pathway blocker group(group G)).After cells were treated for 48 hours,flow cytometry was used to detect the early apoptosis,and RT-PCR was performed to measure the mRNA expression of RhoA and ROCK.Results There were no significant difference in early apoptosis and mRNA expression of RhoA and ROCK between blank control group and ATO group(P>0.05).However,the combined treatment with ATO inhibited the apoptosis and reduced the expression of RhoA and ROCK in albumin-treated cells in a dose-dependent manner(P<0.05).In addition,compared with group B,the apoptosis of HK-2 cells decreased in group G(P<0.05).Conclusion ATO can inhibit albumin-induced early apoptosis in HK-2 cells by blocking RhoA/ROCK signal pathway.

关 键 词：阿托伐他汀 细胞凋亡 肾小管上皮细胞 

分 类 号：R-33[医药卫生] R-34