白脂素对高浓度葡萄糖诱导心肌细胞损伤的保护作用及机制  

Protective Effect of Asprosin on Myocardial Cell Injury Induced by High Glucose and Its Mechanism of Action

作　　者：张雅楠 李浩东 章芯怡 韩杰 黄起壬 ZHANG Ya-nan;LI Hao-dong;ZHANG Xin-yi;HAN Jie;HUANG Qi-ren(Jiangxi Provincial Key Laboratory of Basic Pharmacology;Department of Pharmacology,College of Pharmacy,Nanchang University,Nanchang 330006,China;Grade 2018,College of Pharmacy,Nanchang University,Nanchang 330006,China)

机构地区：[1]江西省基础药理学重点实验室 [2]南昌大学药学院药理学教研室,南昌330006 [3]南昌大学药学院,南昌330006

出　　处：《南昌大学学报（医学版）》2021年第5期24-30,共7页Journal of Nanchang University：Medical Sciences

基　　金：南昌大学“大学生创新创业训练计划”项目(2020CX261)。

摘　　要：目的探讨白脂素(ASP)对高浓度葡萄糖(HG)诱导的心肌细胞(H9C2)损伤的保护作用及机制。方法建立HG诱导H9C2损伤模型。实验分为正常对照组(Ctrl,5.5 mmol·L^(-1))、高浓度葡萄糖组(HG,50 mmol·L^(-1))、Ctrl+ASP(20 nmol·L^(-1))组和HG+ASP(20 nmol·L^(-1))组。Ctrl组和Ctrl+ASP组细胞先用含正常浓度葡萄糖(5.5 mmol·L^(-1))的完全培养基处理36 h,HG组和HG+ASP组则先用含葡萄糖50 mmol·L^(-1)的完全培养基处理36 h,36 h后将各组培养基均换成正常葡萄糖浓度的完全培养基;除Ctrl组和HG组外,其余2组分别加入20 nmol·L^(-1)的ASP继续培养24 h。处理结束后收集细胞和上清液,采用CCK8检测分析各组细胞活力,使用试剂盒检测乳酸脱氢酶(LDH)及丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)等指标,Western blotting和RT-PCR检测各组细胞SOD、GSH-PX蛋白及mRNA表达量,荧光倒置显微镜检测ROS水平。结果当用葡萄糖浓度为50 mmol·L^(-1)的培养基处理细胞时,细胞活力下降,LDH活性升高,与Ctrl组比较,差异有统计学意义(P<0.05),表明损伤模型建立成功;与HG组相比,HG+ASP组的细胞活力升高,LDH活性和MDA含量下降,SOD和GSH-PX的表达和活性均显著升高,ROS水平显著下降(均P<0.05)。结论ASP对HG诱导的H9C2损伤具有保护作用,其机制主要通过对抗ROS介导的氧化应激来实现。Objective To explore the protective effect of asprosin(ASP)on myocardial cell(H9C2)injury induced by high glucose(HG)and its mechanism of action.Methods H9C2 cell damage was induced by HG.Cells were divided into four groups.The control group(Ctrl group)and HG group were given 5.5 mmol·L^(-1)and 50 mmol·L^(-1)glucose for 36 h,respectively.In Ctrl+ASP group and HG+ASP group,cells were challenged with 20 nmol·L^(-1)ASP for 24 h in normal glucose concentration of medium after treatment with 5.5 mmol·L^(-1)and 50 mmol·L^(-1)glucose for 36 h,respectively.At the end of treatment,the cells and supernatant were collected,and cell viability was measured by CCK8 assay.Furthermore,lactate dehydrogenase(LDH),malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX)were detected using the kits.The expression of SOD and GSH-Px and their mRNA was determined by Western blotting and RT-PCR.The production of ROS was observed by inverted fluorescence microscope.ResultsCompared with Ctrl group,treatment with 50 mmol·L^(-1)glucose decreased cell viability and increased LDH activity(P<0.05),indicating that the damage model was established successfully.Compared with HG group,LDH activity,MDA level and ROS content were reduced and cell viability and expression and activity of SOD and GSH-Px were elevated in HG+APS group(P<0.05).Conclusion ASP can protect against HG-induced H9C2 cell injury via inhibiting ROS-mediated oxidative stress.

关 键 词：白脂素 高浓度葡萄糖 心肌细胞 氧化应激 

分 类 号：R965[医药卫生—药理学]