RNAi与转Bt基因技术协同抗虫研究进展  

Review on the Synergistic Insect-resistant Application of RNAi and Bt-transgenic Technologies

作　　者：邓普荣 刘勇波[1] DENG Pu-rong;LIU Yong-bo(State Key Laboratory of Environmental Criteria and Risk Assessment,Chinese Research Academy of Environmental Sciences,Beijing 100012)

机构地区：[1]中国环境科学研究院环境基准与风险评估国家重点实验室,北京100012

出　　处：《生物技术通报》2021年第10期216-224,共9页Biotechnology Bulletin

基　　金：转基因生物新品种培育重大专项(2012ZX08011002)。

摘　　要：转基因抗虫作物降低了昆虫的危害并减少了农药的使用,但是转Bt(Bacillus thuringiensis)基因抗虫作物的大面积连续种植使得靶标害虫对转Bt基因植物产生抗性进化,导致其长期抗虫效果受到挑战。为延缓靶标害虫对Bt蛋白的抗性进化,提高转基因作物的抗虫能力,RNA干扰(RNA interference,RNAi)技术与转Bt基因技术协同应用于农业害虫防治。本文综述了RNAi技术应用于农业害虫防控的进展,以及与转基因抗虫技术联合防治农业害虫的研究进展,分析其面临的挑战和技术难题,以期支撑RNAi与转基因技术发展成一种绿色环保和可持续发展的害虫综合防控技术。The cultivation of transgenic insect-resistance crops has decreased the damage of insects on plants and the application of chemical pesticides in the field.However,the long-term continuous cultivation of Bt-transgenic crops worldwide has increased the resistance evolution of target insects to Bt-transgenic plants in the field,leading its long-term insect resistance to be in challenge.To delay the resistance evolution of target insects to Bt proteins and to improve the insect-resistance of transgenic crops,RNAi and Bt-transgenic biotechnologies have been synergistically applied to control agricultural pests.Here,we review the progresses on RNAi technology applied in agricultural pest-control and on the synergistic application of Bt-transgene technology in agricultural pest-control,and discuss the challenges and technological difficulties,aiming to promote the combined application of RNAi and transgenic technologies to be environment-friendly and sustainable in controlling agricultural pests.

关 键 词：双链RNA RNA干扰 转基因作物 害虫防治 

分 类 号：S332.3[农业科学—作物遗传育种]