紫花苜蓿CCoAOMT基因家族的鉴定、进化及表达分析  

Identification,evolution and expression analysis of the CCoAOMT family genes in Medicago sativa

作　　者：马倩 闫启[1] 张正社[1] 吴凡[1] 张吉宇[1] MA Qian;YAN Qi;ZHANG Zheng-she;WU Fan;ZHANG Ji-yu(State Key Laboratory of Grassland Agro-ecosystems,Key Laboratory of Grassland Livestock Industry Innovation,Ministry of Agriculture and Rural Affairs,Engineering Research Center of Grassland Industry,Ministry of Education,College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China)

机构地区：[1]兰州大学草地农业生态系统国家重点实验室,兰州大学农业农村部草牧业创新重点实验室,兰州大学草地农业教育部工程研究中心,兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业学报》2021年第11期144-156,共13页Acta Prataculturae Sinica

基　　金：中国科学院战略性先导科技专项(XDA26030103-3);甘肃省科技重大专项计划课题(19ZD2NA002)资助。

摘　　要：紫花苜蓿是全世界广泛栽培的优质牧草。木质素与紫花苜蓿抗性密切相关,但木质素难以被牲畜消化,严重影响了紫花苜蓿的营养价值,所以培育低木质素的紫花苜蓿具有重要意义。咖啡酰辅酶A氧甲基转移酶(CCoAOMT)是木质素合成途径中的关键酶,本研究利用生物信息学方法对紫花苜蓿基因组中的CCoAOMT基因家族成员进行鉴定,并对其基因结构、染色体定位、系统发育、基因表达等方面进行了研究。结果表明,在紫花苜蓿基因组中共鉴定到44个MsCCoAOMT基因,其中36个(82%)MsCCoAOMT基因都含有5个外显子,分布于16条染色体上,存在明显的串联重复现象;通过系统发育树分析,MsCCoAOMT基因家族可分为5类;通过MEME软件对MsCCoAOMT蛋白motif进行预测发现了10个比较保守的motif。qRT-PCR表达分析表明部分MsCCoAOMT基因的表达具有组织特异性。研究结果对紫花苜蓿低木质素遗传改良具有潜在的理论指导意义和实际参考价值。Alfalfa(Medicago sativa)is a forage legume with high feed quality widely cultivated all over the world. It contains lignins that play an important role in stress resistance of alfalfa. However,lignin seriously affects the nutritional value of alfalfa because it is difficult for livestock to digest. Therefore,it is necessary to breed alfalfa varieties with low lignin content. Caffeoyl CoA O-methyltransferase(CCoAOMT)is a key enzyme in the lignin synthesis pathway. In this study,the members of CCoAOMT gene family in the alfalfa genome were identified by bioinformatic methods, and the gene structure, chromosome location, phylogeny and gene expression were analysed. It was found that there were 44 MsCCoAOMT genes in the alfalfa genome. These genes were distributed on 16 chromosomes with tandem duplication,among which 36(82%)MsCCoAOMT genes contained 5 exons. A phylogenetic tree was constructed and showed that MsCCoAOMT family genes can be classified into 5 groups. In addition,ten conserved motifs were found by predicting the motif of MsCCoAOMT protein with MEME software.qRT-PCR results indicated that most of MsCCoAOMT genes were tissue-specific in their expression. Our results provide reference information for the genetic improvement of alfalfa and development of lines with low lignin content.

关 键 词：紫花苜蓿 木质素 咖啡酰辅酶A氧甲基转移酶 基因家族 基因表达 

分 类 号：S541.9[农业科学—作物学]