基于网络药理学探讨通冠胶囊治疗动脉粥样硬化的作用机制  

Investigation on Action Mechanism of Tongguan Capsules for Atherosclerosis Based on Network Pharmacology

作　　者：李锦伟 魏启明 朱晓峰 孟凡喆[1] 常钢[1] 刘文导[1] LI Jinwei;WEI Qiming;ZHU Xiaofeng;MENG Fanzhe;CHANG Gang;LIU Wendao

机构地区：[1]广东省中医院,广东广州510120

出　　处：《新中医》2021年第20期1-6,共6页New Chinese Medicine

基　　金：广东省科技厅科技计划项目(粤科规财字[2017]103号)。

摘　　要：目的:利用网络药理学方法探讨通冠胶囊对动脉粥样硬化性疾病的作用机制。方法:采用TCMSP数据库和文献挖掘方法对通冠胶囊的药物化合物及靶点进行筛选;通过GeneCards及DisGeNET数据库检索动脉粥样硬化性疾病的相关靶点;通过Cytoscape 3.8.0软件构建通冠胶囊药物-靶点-疾病网络及核心网络,通过STRING数据库构建其PPI网络。截取通冠胶囊作用于动脉粥样硬化性疾病潜在靶点于DAVID网站进行KEGG及GO富集分析。采用R软件对将上述信号通路、生物学过程、分子功能、细胞组成信息进行可视化处理。结果:共获得通冠胶囊化合物123个,通冠胶囊药物靶点272个,对应化合物111个;动脉粥样硬化性疾病靶点576个。形成通冠胶囊药物与动脉粥样硬化靶点交集图,共有NR3C1、DPP4、ESR1、MAPK14、PPARG、PTGS2等61个共同靶点,相应化合物101个。PPI网络中共得到56个相互连接的节点,288条边。KEGG与GO分析后得到通冠胶囊可能作用于动脉粥样硬化的NOD样受体信号通路、癌症的途径、VEGF信号通路等22条通路。结论:通冠胶囊可通过作用于NR3C1、DPP4、ESR1、MAPK14、PPARG、PTGS2等61个共同靶点,调节胰岛素信号通路,发挥抑制炎症反应,调节代谢,抗氧化应激等作用,干预动脉粥样硬化发生及发展进程。Objective:To investigate the action mechanism of Tongguan capsules for atherosclerotic diseases by means of network pharmacology.Methods:Pharmaceutical compounds and targets of Tongguan capsules were screened by TCMSP database and literature mining;the related targets of atherosclerotic diseases were retrieved through GeneCards and DisGeNET databases;a Tongguan capsules-target-disease network and a core network were constructed through Cytoscape 3.8.0 software and its PPI network was constructed through STRING database.Potential targets of Tongguan capsules for atherosclerotic diseases were selected for KEGG and GO enrichment analysis on DAVID website.R software was applied to visualize the above signal pathway,biological process,molecular function and cell composition information.Results:A total of 123 compounds,272 drug targets and 111 corresponding compounds of Tongguan capsules were obtained;there were 576 targets of atherosclerotic diseases.The intersection graph of drug targets of Tongguan capsules and targets of atherosclerosis was formed,with a total of 61 common targets such as NR3 C1,DPP4,ESR1,MAPK14,PPARG and PTGS2,and 101 corresponding compounds.There were 56 interconnected nodal points and 288 edges in PPI network.After KEGG and GO analysis,22 pathways for Tongguan capsules to act on atherosclerosis were obtained,including NOD-like receptor signaling pathway,cancer pathway and VEGF signaling pathway.Conclusion:Tongguan capsules can regulate insulin signaling pathway,play a role in inhibiting inflammatory responses,regulating metabolism and anti-oxidative stress as well as intervene in the occurrence and development of atherosclerosis by acting on 61 common targets such as NR3 C1,DPP4,ESR1,MAPK14,PPARG and PTGS2.

关 键 词：动脉粥样硬化 网络药理学 通冠胶囊 作用机制 

分 类 号：R543.5[医药卫生—心血管疾病]