隔药饼灸对动脉粥样硬化兔血清脂联素与血脂水平的影响  

Effect of Herbal Cake-separated Moxibustion on Serum Adiponectin and Blood Lipid Levels in Rabbits with Atherosclerosis

作　　者：方园 黄河[1] 刘迈兰[1] 刘红华[1] 冯芳 常小荣[1] FANG Yuan;HUANG He;LIU Mailan;LIU Honghua;FENG Fang;CHANG Xiaorong(Hunan University of Chinese Medicine,Changsha,Hunan 410000,China;West Hospital of Chenzhou First People’s Hospital,Chenzhou,Hunan 423000,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]郴州第一人民医院西院,湖南郴州423000

出　　处：《湖南中医药大学学报》2021年第10期1477-1481,共5页Journal of Hunan University of Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2015CB554502);国家自然科学基金项目(81574082,81874509);国家自然科学基金面上项目(82074559);国家中医药管理局全国名老中医药专家传承工作室项目(国中医药人教函〔2019:41号);常小荣国家级教学名师工作室建设项目;湖南省教育厅优秀青年基金项目(19B435);湖南省中医药科研计划课题项目(201964);湖南省教育厅优秀青年基金项目(19B428);湖南省研究生科研创新项目(2020CX28);长沙市杰出创新青年培养计划项目(kq1905036);湖南中医药大学第一附属医院湖南省院士专家工作站(石学敏)开放基金(2019YSZJJ)。

摘　　要：目的观察隔药饼灸对动脉粥样硬化兔血清脂联素及血脂含量的影响。方法将40只雄性新西兰兔随机分为正常组、模型组、隔药饼灸组、阿托伐他汀组,每组10只,模型组喂食高胆固醇饲料,阿托伐他汀组予高胆固醇饲料加阿托伐他汀喂养,隔药饼灸组喂食高胆固醇饲料同时予以隔药饼灸,12周后取材。采用ELISA法测定各组兔血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)及血清脂联素(ADPN)含量;HE染色观察兔主动脉管壁形态结构。结果干预后,与正常组比较,模型组TC、TG、LDL-C均明显升高(P<0.01),ADPN水平明显降低(P<0.01)。与模型组比较,隔药饼灸组、阿托伐他汀组TC、TG、LDL-C均不同程度降低(P<0.05,P<0.01),隔药饼灸组ADPN含量升高(P<0.05),阿托伐他汀组ADPN水平升高,但差异无统计学意义(P>0.05)。与正常组比较,模型组主动脉管壁结构明显受损;与模型组比较,隔药饼灸组、阿托伐他汀组主动脉内皮结构明显改善。结论隔药饼灸抗动脉粥样硬化的作用机制,可能与调节血清ADPN含量、降低血脂水平、改善动脉管壁损伤情况以及防治动脉粥样硬化斑块的形成有关。Objective To observe the effect of herbal cake-separated moxibustion on serum adiponectin and lipid content of atherosclerotic rabbits.Methods 40 male New Zealand rabbits were randomly divided into normal group,model group,herbal cake-separated moxibustion group and atorvastatin group,with 10 rabbits in each group.The model group was fed with high-cholesterol diet,the atorvastatin group was fed with high-cholesterol diet plus atorvastatin,and the herbal cake-separated moxibustion group was fed with high-cholesterol diet and given herbal cake-separated moxibustion,and samples were taken after 12 weeks.The content of serum total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C)and serum adiponectin(ADPN)were measured by ELISA.HE staining was used to observe the morphology and structure of rabbit aortic wall.Results After intervention,TC,TG and LDL-C in the model group were significantly higher than those in the normal group(P<0.01),the serum ADPN level in the model group decreased significantly(P<0.01).Compared with the model group,TC,TG and LDL-C in herbal cake-separated moxibustion group and atorvastatin group were all decreased to different degrees(P<0.01,P<0.05),the serum ADPN content in the herbal cake-separated moxibustion group increased(P<0.05),the serum ADPN level in atorvastatin group increased,but the difference was not statistically significant(P>0.05).Compared with the normal group,the aortic wall structure of the model group was significantly damaged,and compared with the model group,the aortic endothelial structure of the herbal cake-separated moxibustion group and atorvastatin group was significantly improved.Conclusion The anti-atherosclerotic mechanism of herbal cake-separated moxibustion may be related to regulating the content of serum ADPN,reducing the level of blood lipid,improving the injury of arterial wall,and preventing and treating the formation of atherosclerotic plaque.

关 键 词：动脉粥样硬化 隔药饼灸 脂联素 总胆固醇 甘油三酯 低密度脂蛋白胆固醇 

分 类 号：R245.8[医药卫生—针灸推拿学]