TRPV1参与针和灸不同刺激方式对小鼠ghrelin和GHSR-1α的影响  

Effect of TRPV1 on ghrelin and GHSR-1αin Mice by Participating in Different Stimulation Methods of Acupuncture and Moxibustion

作　　者：刘琼[1] 曹佳男 刘密[1] 谢辉[2] 常小荣[1] LIU Qiong;CAO Jia’nan;LIU Mi;XIE Hui;CHANG Xiaorong(Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;West Hospital of Chenzhou First People's Hospital,Chenzhou,Hunan 423000,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]郴州市第一人民医院西院,湖南郴州423699

出　　处：《湖南中医药大学学报》2021年第10期1487-1492,共6页Journal of Hunan University of Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2015CB554502);院士工作站(石学敏)开放基金项目(2019YSZJJ13);国家自然科学基金项目(81874509);国家中医药管理局全国名老中医药专家传承工作室(国中医药人教函2019:41号);常小荣国家级教学名师工作室建设项目。

摘　　要：目的通过观察针刺或热灸不同刺激方式干预足三里穴区对C57BL/6野生型小鼠和TRPV1^(-/-)基因敲除小鼠血浆中脑肠肽(ghrelin)和大脑前额叶皮质中生长激素促分泌素受体-1α(GHSR-1α)含量的改变,探讨辣椒素受体1(TRPV1)参与针、灸不同刺激方式对胃肠功能调控通路ghrelin-GHSR-1α调节的差异性。方法24只C57BL/6野生型小鼠随机分为C57BL/6空白组、C57BL/6针刺组和C57BL/6热灸组,每组8只;24只TRPV1^(-/-)基因敲除小鼠随机分为TRPV1^(-/-)空白组、TRPV1^(-/-)针刺组和TRPV1^(-/-)热灸组,每组8只。C57BL/6空白组和TRPV1^(-/-)空白组不予以处理;C57BL/6针刺组和TRPV1^(-/-)针刺给予手针干预;C57BL/6热灸组和TRPV1^(-/-)热灸组给予热灸干预。采用ELISA检测小鼠血浆中ghrelin含量和Western Blot法检测小鼠大脑前额叶皮质中TRPV1和GHSR-1α表达水平的变化。结果C57BL/6野生型小鼠中,与C57BL/6空白组相比,C57BL/6针刺组与热灸组血浆ghrelin表达水平均显著降低(P<0.05),前额叶皮层TRPV1含量表达增加(P<0.05)、GHSR-1α降低(P<0.05),但三者在针刺组和热灸组间差异无统计学意义(P>0.05)。TRPV1^(-/-)基因敲除小鼠中,与TRPV1^(-/-)空白组对比,TRPV1^(-/-)针刺组和热灸组血浆ghrelin水平和前额叶皮质GHSR-1α表达水平差异均无统计学意义(P>0.05)。结论针刺、热灸均可通过激活TRPV1的生物活性,影响胃肠功能调控通路ghrelin-GHSR-1α的调节作用。Objective To observe the effect of different stimulation modalities of acupuncture or pyretic moxibustion in the“Zusanli”(ST36)acupoint on changes of content of plasma ghrelin and growth hormone secretagogue receptor-1(GHSR-1α)in the prefrontal cortex of C57BL/6 wild-type mice and TRPV1^(-/-)knockout mice,and to explore the difference of capsaicin receptor 1(TRPV1)involved in acupuncture and pyretic moxibustion stimulation on ghrelin-GHSR-1αregulation of gastrointestinal function pathway.Methods 24 C57BL/6 wild-type mice were randomly divided into C57BL/6 blank group,C57BL/6 acupuncture group and C57BL/6 hot pyretic moxibustion group,with 8 mice in each group.24 TRPV1^(-/-)knockout mice were randomly divided into TRPV1^(-/-)blank group,TRPV1^(-/-)acupuncture group and TRPV1^(-/-)pyretic moxibustion group,with 8 mice in each group.C57BL/6 blank group and TRPV1^(-/-)blank group were not treated;C57BL/6 acupuncture group and TRPV1^(-/-)acupuncture group were given hand acupuncture intervention;C57BL/6 pyretic moxibustion group and TRPV1^(-/-)pyretic moxibustion group were given pyretic moxibustion intervention.ELISA was used to detect the ghrelin content in mouse plasma,and Western blot was used to detect the changes of TRPV1 and GHSR-1αexpression levels in mouse brain prefrontal cortex.Results In C57BL/6 wild-type mice,compared with the C57BL/6 blank group,plasma ghrelin level in C57BL/6 acupuncture group and pyretic moxibustion group was significantly decreased(P<0.05),while the content of TRPV1 in prefrontal cortex was increased(P<0.05)and GHSR-1αwas decreased(P<0.05),but there was no statistically significant difference between the acupuncture group and pyretic moxibustion group(P>0.05).In TRPV1^(-/-)knockout mice,compared with the TRPV1^(-/-)blank group,there was no statistically significant difference in plasma ghrelin level and prefrontal cortex GHSR-1αexpression level in TRPV1^(-/-)acupuncture group and pyretic moxibustion group(P>0.05).Conclusion Both acupuncture and pyretic moxibustion can affect

关 键 词：针刺 热灸 足三里 脑肠肽 生长激素促分泌素受体-1α 辣椒素受体1 

分 类 号：R245.8[医药卫生—针灸推拿学]