D型Fmoc-苯丙氨酸/透明质酸复合双网络水凝胶的制备及应用  

Preparation and application of Fmoc-^(D)phenylalanine/hyaluronic acid composite double-network hydrogel

作　　者：付成 刘敏 鲁望婷 袁发浒[2] 李雯慧 邓云 FU Cheng;LIU Min;LU Wangting;YUAN Fahu;LI Wenhui;DENG Yun(Institute for Interdisciplinary Research,Jianghan University,Wuhan 430056,China;School of medicine,Jianghan University,Wuhan 430056,China)

机构地区：[1]江汉大学交叉学科研究院,武汉430056 [2]江汉大学医学院,武汉430056

出　　处：《复合材料学报》2021年第10期3443-3453,共11页Acta Materiae Compositae Sinica

基　　金：湖北省教育厅科学技术研究项目(B2019237)。

摘　　要：将N-芴甲氧羰基-D-苯丙氨酸(Fmoc-^(D)Phe)和甲基丙烯酸缩水甘油酯(Glycidyl methacrylate,GMA)修饰的透明质酸(HA-GMA)在磷酸缓冲液中共混加热,冷却后Fmoc-^(D)Phe分子先自组装形成超分子水凝胶,超分子水凝胶中的HA-GMA再经光照引发交联制备双网络复合水凝胶。研究该双网络水凝胶的力学性能、光学性质、微观形貌、药物缓释能力和抑菌性能。研究结果表明,双网络水凝胶比HA-GMA单网络水凝胶的力学性能强一倍左右且HA-GMA网络存在于双网络水凝胶中;光学性质显示双网络水凝胶中存在Fmoc-^(D)Phe网络;微观形貌表明有两种水凝胶网络均存在于复合水凝胶中。当复合水凝胶包裹小分子模拟药物后,复合水凝胶达到模拟药物最大累积释放量的时间要比Fmoc-^(D)Phe单网络水凝胶的长6 h;针对革兰氏阳性细菌的抑菌能力研究显示,双网络水凝胶的抑菌效果也比Fmoc-^(D)Phe单网络水凝胶的更好。N-fluoren-methoxycarbonyl-D-Phenylalanine(Fmoc-^(D)Phe) and glycidyl methacrylate(GMA)-modified hyaluronic acid(HA-GMA) were mixed and heated in phosphoric acid buffer. After cooling, Fmoc-^(D)Phe molecules were self-assemble into supermolecular hydrogel followed by forming double network hydrogel with HA-GMA under UV light irradiation. The mechanical properties, optical properties, microstructure, drug release and bacteriostatic properties of the composite hydrogel were studied. The results show that the double-network hydrogels are about twice as strong as those of HA-GMA single-network hydrogel, and the HA-GMA network exists in the doublenetwork hydrogels. Optical properties show the existence of Fmoc-^(D)Phe network in the double network hydrogels.The microstructure indicates that there are two kinds of hydrogel networks in the composite hydrogel. Moreover,after loading the drug, the double-network hydrogel takes 6 hours longer to reach the maximum drug accumulation rate than the Fmoc-^(D)Phe single-network hydrogel. The antibacterial activity of the double-network hydrogel against gram-positive bacteria is also better than that of the Fmoc-^(D)Phe single-network hydrogel.

关 键 词：D型Fmoc-苯丙氨酸 透明质酸 复合水凝胶 药物释放 抑菌性能 

分 类 号：TQ427.26[化学工程]