脂多糖对大黄鱼原代头肾巨噬细胞的激活作用  

The activation of lipopolysaccharide in primary head kidney macrophages of large yellow croaker

作　　者：程安怡 赵金鹏 黄小红[1] 张伟妮[1,2] CHENG Anyi;ZHAO Jinpeng;HUANG Xiaohong;ZHANG Weini(University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Key Laboratory of Marine Biotechnology of Fujian Province,Institute of Oceanology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学中西兽医结合与动物保健福建省高校重点实验室,福建福州350002 [2]福建农林大学海洋研究院福建省海洋生物技术重点实验室,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2021年第6期805-811,共7页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金资助项目(31802333);福建农林大学科技创新专项基金项目(CXZX2019065S)。

摘　　要：为了探究脂多糖(LPS)对大黄鱼原代头肾巨噬细胞(PKM)的激活作用及其相应受体,采用流式细胞术检测LPS对大黄鱼PKM吞噬活性和氮呼吸爆发的影响,用实时荧光定量PCR检测LPS对3种促炎因子(IL-1β、IL-6、IL-8)和7种受体基因(TLR1、TLR2、TLR21、MR1、MR2、NOD1、NOD2)转录水平的影响.结果显示:0.1μg·mL^(-1)LPS可以显著提高大黄鱼PKM的吞噬能力(P<0.05),1、10、100μg·mL^(-1)LPS可以极显著地增强细胞的吞噬能力(P<0.01);1、10、100μg·mL^(-1)LPS可以极显著提高细胞的氮呼吸爆发(P<0.01);LPS可以显著上调3种促炎因子(IL-1β、IL-6、IL-8)和6种受体基因(TLR1、TLR2、TLR21、MR2、NOD1、NOD2)的表达水平,显著下调MR1的表达水平.研究表明,LPS对大黄鱼PKM有明显的激活作用,并刺激细胞向M1型巨噬细胞分化.In order to study the induction of lipopolysaccharide(LPS) in primary head kidney-derived macrophages(PKM) from large yellow croaker(Larimichthys crocea) and its receptors, the effects of LPS on the phagocytic activity and nitrogen respiratory burst of PKM were investigated using flow cytometry method. The effects of LPS on the transcriptional levels of 3 pro-inflammatory cytokines, IL-1β, IL-6 and IL-8, and 7 receptor genes, TLR1, TLR2, TLR21, MR1, MR2, NOD1 and NOD2 were assessed via fluorescent quantitative PCR. The results showed that the phagocytic capacity of L.crocea PKM was enhanced by 0.1 μg·mL^(-1) LPS(P<0.05), and significantly enhanced by 1, 10 and 100 μg·mL^(-1) LPS(P<0.01). Nitrogen respiratory burst level of PKM was improved by LPS at concentrations of 1, 10 and 100 μg·mL^(-1) LPS(P<0.01). Moreover, the gene expression levels of 3 pro-inflammatory cytokines and 6 receptor genes were up-regulated by LPS while the expression of MR1 was down-regulated. To summarize, LPS could significantly activate the PKM of L.crocea, and stimulate the differentiation of PKM into M1-type macrophage.

关 键 词：脂多糖 头肾巨噬细胞 吞噬活性 呼吸爆发 受体 

分 类 号：S917.4[农业科学—水产科学]