不同产地人参组成四君子汤治疗脾胃气虚证的临床观察及其物质基础初探  

Clinical Observation on the Therapeutic Effect and Preliminary Exploration of Material Basis of Ginseng in Sijunzi Decoction in the Treatment of Syndrome of Deficiency of Spleen and Stomach Qi

作　　者：王萌萌[1] 王超众 杜洪波[1] WANG Mengmeng;WANG Chaozhong;DU Hongbo(Department of Integrated Traditional Chinese and Western Medicine,Qiqihar First Hospital,Heilongjiang Province,Qiqihar 161005,China;Qiqihar Food and Drug Inspection and Testing Center,Heilongjiang Province,Qiqihar 161005,China)

机构地区：[1]齐齐哈尔市第一医院中西医结合一科,黑龙江齐齐哈尔161005 [2]齐齐哈尔市食品药品检验检测中心,黑龙江齐齐哈尔161005

出　　处：《光明中医》2021年第19期3209-3211,共3页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：齐齐哈尔市科技计划项目(No.SFZD-2019027);黑龙江省自然科学基金优秀青年项目(No.YQ2019H034);黑龙江省中医药管理局科技计划项目(No.ZHY2020-009);齐齐哈尔市科技计划创新激励项目(No.CSFGG-2020220)。

摘　　要：目的观察不同产地人参组成四君子汤治疗脾胃气虚证的临床疗效,测定不同产地人参组成四君子汤的主要成分含量,从而探究四君子汤的物质基础。方法将110例脾胃气虚证患者随机分为3组,观察1组、观察2组各36例患者,对照组38例患者,观察临床疗效。含量测定部分:采用超声提取法对人参进行提取,再采用水饱和正丁醇对人参提取溶液进行萃取,用紫外可见分光光度法测定其吸光度值。结果观察1组和观察2组疗效均优于对照组,且观察1组治疗脾胃气虚证的临床疗效优于观察2组(P<0.05),差异具有统计学意义;检测吉林产地的人参与山西产地的人参均含有人参皂苷成分,其中吉林产地的人参配制的四君子汤人参总皂苷含量为1367μg/ml,山西产地的人参配制的四君子汤人参总皂苷含量为778μg/ml,吉林产地的人参配制的四君子汤人参皂苷含量明显高于山西产地的人参配制的四君子汤。结论推测人参总皂苷是四君子汤治疗脾胃气虚证的物质基础。Objective To observe the clinical effect of ginseng in Sijunzi decoction in the treatment of syndrome of spleen and stomach qi deficiency,and to determine the content of the main components of ginseng in Sijunzi decoction in different areas,so as to explore the material basis of Sijunzi decoction.Methods Clinical observation:110 patients with syndrome of spleen and stomach qi deficiency were randomly divided into observation group one,with two observation groups of 36 patients respectively,and control group with 38 patients.The clinical effect was observed.The content determination part:the ultrasonic extraction method was used to extract the ginseng,and then the water saturated n-butanol was used to extract the ginseng extract solution,and the absorbance value was determined by UV spectrophotometry.Results The curative effect of observation group one and observation group two was better than that of control group.Furthermore,the clinical effect of spleen-stomach qi deficiency in group one was better than that in group two(P<0.05),and there had statistical significance.The ginseng from Jilin producing area and from Shanxi producing area all contains ginsenoside.The total ginsenosides content of Sijunzi decoction prepared by ginseng from Jilin origin was 1367μg/ml,and the total ginsenosides content of Sijunzi decoction prepared by ginseng from Shanxi origin was 778μg/ml.The content of ginsenoside in Sijunzi decoction prepared by ginseng from Jilin origin was significantly higher than that prepared by ginseng from Shanxi origin.Conclusion It is speculated that ginseng total saponins is Sijunzi decoction material basis for the treatment of spleen deficiency syndrome.

关 键 词：人参 四君子汤 脾胃气虚证 紫外-可见分光光度法 人参总皂苷 物质基础 

分 类 号：R289.5[医药卫生—方剂学]