鼠麴草对大鼠佐剂性关节炎的免疫调节作用  

Immunoregulatory effect of Gnaphalium affine D.Don on adjuvant arthritis in rats

作　　者：黄蕾[1] 杨全伟[1] 刘新国[1] 文雯 胡松[1] HUANG Lei;YANG Quanwei;LIU Xinguo;WEN Wen;HU Song(Department of Pharmacy,Wuhan No.1 Hospital,Wuhan 430022;Pharmaceutical Company,Grandpharma(China)Co.Ltd.,Wuhan 430040)

机构地区：[1]武汉市第一医院药学部,湖北武汉430022 [2]远大医药有限公司制剂分公司,湖北武汉430040

出　　处：《湖北中医药大学学报》2021年第5期17-21,共5页Journal of Hubei University of Chinese Medicine

基　　金：湖北省卫生与计划生育委员会资助项目(项目编号:WJ2017M180)。

摘　　要：目的研究鼠麴草对大鼠佐剂性关节炎(AA)的抗炎消肿作用、相关炎症因子以及大鼠免疫功能的影响。方法采用注射弗氏完全佐剂(FCA)进行AA大鼠造模,造模成功的大鼠随机分为正常组、模型组和鼠麹草低、中、高剂量组、雷公藤多苷组。考察AA大鼠关节足胀度及胸腺、脾脏指数,采用酶联免疫吸附法(ELISA)测定大鼠血清中的前列腺素E2(PGE2)、肿瘤坏死因子(TNF-α)及白细胞介素1β(IL-1β)的水平流式细胞术(FCM)检测大鼠脾脏T淋巴细胞亚群的比例。结果与模型组比较,鼠麴草低、中、高剂量组均可以显著降低大鼠踝关节肿胀率(P<0.05,P<0.01),大鼠血清中PGE2、IL-1β、TNF-α的含量明显降低(P<0.01),脾脏系数、胸腺系数明显降低,差异有统计学意义(P<0.05,P<0.01),可显著抑制AA大鼠脾脏CD4^(+),CD8^(+)淋巴细胞的增殖(P<0.05 P<0.01)升高CD4^(+)/CD8^(+)细胞比例。结论鼠麴草能抑制AA模型大鼠的足肿胀度,降低血清中炎症细胞因子水平,并改善异常的免疫功能,其机制可能与其调节CD4^(+)/CD8^(+)的平衡有关。Objective To study the anti-inflammatory and swelling effects of Gnaphalium affine D.Don on adjuvant arthritis(AA)related inflammatory factors and immune function in rats.Methods AA rats were modeled by injection of Freund’s complete adjuvant(FCA,5 mg/mL)0.05 mL.Rats with successful modeling were randomly divided into normal control group,model group,and Gnaphalium affine D.Don low-dose group,medium-dose group,high-dose group,tripterygium glycoside group.The swelling of articular feet thymus and spleen index of AA rats were ihvestigated,the level of prostaglandin E2(PGE2),tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in serum were determined by enzyme-linked immunosorbent assay(ELISA)flow cytometry(FCM)was used to detect the proportion of T lymphocyte subsets in spleen.Results Compared with model group,the ankle joint swelling rate of rats low,medium,and high dose groups of Gnaphalium affine D.Don was significantly reduced in(P<0.05 P<0.01),the content of PGE_(2) IL-1β,TNF-αin rat serum was significantly reduced(P<0.01),spleen coefficient and thymus coefficient were significantly reduced,the difference was statistically significant(P<0.05 P<0.01),which could significantly inhibit the proliferation of CD4^(+)and CD8^(+)lymphocytes in spleen of AA rats(P<0.05 P<0.01),increasing the ratio of CD4^(+)/CD8^(+)cells.Conclusion Gnaphalium affine D.Don can inhibit the swelling of the feet reduce the level of serum inflammatory cytokines,and improve the abnormal immune function in AA rats.The mechanism may be related to its balance of regulating CD4^(+)/CD8^(+).

关 键 词：佐剂性关节炎 鼠麴草 炎症因子 免疫功能 

分 类 号：R285.5[医药卫生—中药学]