p66Shc-mediated oxidative stress is involved in gestational diabetes mellitus  

作　　者：Ting-Ting Huang Wen-Juan Sun Hai-Ying Liu Hong-Li Ma Bao-Xia Cui 

机构地区：[1]Cheeloo College of Medicine,Shandong University,Jinan 250000,Shandong Province,China [2]Department of Obstetrics,Taian City Central Hospital,Taian 271000,Shandong Province,China [3]Department of Obstetrics,The Second Hospital,Cheeloo College of Medicine,Shandong University,Jinan 250000,Shandong Province,China [4]Department of Obstetrics and Gynecology,Qilu Hospital(Qingdao),Cheeloo College of Medicine,Shandong University,Qingdao 266000,Shandong Province,China [5]Department of Obstetrics and Gynecology,Qilu Hospital,Cheeloo College of Medicine,Shandong University,Jinan 250013,Shandong Province,China

出　　处：《World Journal of Diabetes》2021年第11期1894-1907,共14页世界糖尿病杂志（英文版）（电子版）

基　　金：The Scientific Research Fund of Qilu Hospital(Qingdao),No.QDKY2015ZD04.

摘　　要：BACKGROUND Gestational diabetes mellitus(GDM)is associated with a heightened level of oxidative stress,which is characterized by the overproduction of reactive oxygen species(ROS)from mitochondria.Previous studies showed that mitochondrial dysfunction is regulated by dynamin-related protein 1(Drp1)and p66Shc in GDM.AIM The aim was to investigate the expression of Drp1 and p66Shc and their possible mechanisms in the pathogenesis of GDM.METHODS A total of 30 pregnant women,15 with GDM and 15 without GDM,were enrolled.Peripheral blood mononuclear cells and placental tissue were collected.The human JEG3 trophoblast cell line was cultivated in 5.5 mmol/L or 30 mmol/L glucose and transfected with wild-type(wt)-p66Shc and p66Shc siRNA.P66Shc and Drp1 mRNA levels were detected by quantitative real-time polymerase chain reaction.The expression of p66Shc and Drp1 was assayed by immunohistochemistry and western blotting.ROS was assayed by dihydroethidium staining.RESULTS The p66Shc mRNA level was increased in the serum(P<0.01)and placentas(P<0.01)of women with GDM,and the expression of Drp1 mRNA and protein were also increased in placentas(P<0.05).In JEG3 cells treated with 30 mmol/L glucose,the mRNA and protein expression of p66Shc and Drp1 were increased at 24 h(both P<0.05),48 h(both P<0.01)and 72 h(both P<0.001).ROS expression was also increased.High levels of Drp1 and ROS expression were detected in JEG3 cells transfected with wt-p66Shc(P<0.01),and low levels were detected in JEG3 cells transfected with p66Shc siRNA(P<0.05).CONCLUSION The upregulated expression of Drp1 and p66shc may contribute to the occurrence and development of GDM.Regulation of the mitochondrial fusion-fission balance could be a novel strategy for GDM treatment.

关 键 词：P66SHC Dynamin-related protein 1 Gestational diabetes mellitus Oxidative stress Mitochondrial dysfunction 

分 类 号：R714.256[医药卫生—妇产科学]