p-香豆酸通过加速伤口处聚酚软木脂和木质素的沉积促进马铃薯块茎愈伤  

p-Coumaric Acid Promoted Wound Healing of Potato Tubers by Accelerating the Deposition of Suberin Poly Phenolic and Lignin at Wound Sites

作　　者：梁伟 朱亚同 柴秀伟 孔蕊 李斌山 李永才 毕阳[1] DOV Prusky LIANG Wei;ZHU YaTong;CHAI XiuWei;KONG Rui;LI BinShan;LI YongCai;BI Yang;DOV Prusky(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China;Department of Postharvest Science of Fresh Produce,Agricultural Research Organization,Rishon LeZion 7505101,Israel)

机构地区：[1]甘肃农业大学食品科学与工程学院,中国兰州730070 [2]Department of Postharvest Science of Fresh Produce,Agricultural Research Organization,Rishon LeZion 7505101,Israel

出　　处：《中国农业科学》2021年第20期4434-4445,共12页Scientia Agricultura Sinica

基　　金：国家自然科学基金(31772040);甘肃省马铃薯产业技术体系(GARS-MLS-S)。

摘　　要：【目的】研究p-香豆酸(p-Coumaric acid,p-CA)处理对马铃薯块茎伤口处聚酚软木脂和木质素积累的影响及相关机理。【方法】用0.5 mmol·L^(-1) p-CA浸泡切半‘大西洋’马铃薯块茎10 min,以蒸馏水处理作为对照,于室温条件下避光贮存进行愈伤。测定损伤块茎在愈伤期间的失重率以及损伤接种硫色镰刀菌(Fusarium sulphureum)块茎的病情指数,观察伤口处聚酚软木脂和木质素的积累,测定伤口处苯丙烷代谢关键酶和过氧化物酶活性,以及苯丙烷代谢产物和H_(2)O_(2)含量。【结果】p-CA处理显著降低了损伤块茎的失重率和损伤接种块茎的病情指数,14 d时,处理块茎的失重率和病情指数分别比对照低38.46%和43.18%。p-CA处理加速了块茎伤口处聚酚软木脂和木质素的积累,14 d时,处理块茎的聚酚软木脂和木质素的细胞层厚度分别比对照高26.43%和30.26%。p-CA处理还明显提高了块茎伤口处苯丙氨酸解氨酶、肉桂酸-4-羟化酶、4-香豆酰辅酶A连接酶和肉桂醇脱氢酶的活性,7 d时,处理块茎的酶活性分别比对照高32.17%、23.51%、25.09%和23.08%。14 d时,p-CA处理块茎的肉桂酸、p-香豆酸、咖啡酸和总酚含量分别比对照高34.26、38.29%、19.31%和41.04%;21 d时,处理块茎的阿魏酸、芥子酸含量分别比对照高38.33%和20.47%。此外,p-CA处理促进了块茎伤口处肉桂醇、松柏醇、芥子醇和木质素的积累,14 d时,处理块茎的肉桂醇和木质素含量分别高于对照36.93%和31.66%;21 d时,处理块茎的松柏醇和芥子醇含量分别高于对照41.43%和34.05%。p-CA处理还提高了块茎伤口处H_(2)O_(2)含量和过氧化物酶活性,7 d时,处理块茎的H_(2)O_(2)含量和过氧化物酶活性分别高于对照40.25%和27.01%。【结论】p-CA处理可通过激活苯丙烷代谢,提高H_(2)O_(2)含量和过氧化物酶活性,促进马铃薯块茎伤口处聚酚软木脂和木质素的积累,从而加速块茎愈伤。【Objective】This study aimed to investigate the effect and related mechanism of p-CA treatment on the accumulation of suberin polyphenolic and lignin at wound sites of potato tubers.【Method】The half cut potato tubers‘cv.Atlantic’were soaked with 0.5 mmol·L^(-1) p-CA for 10 min(using distilled water treated as control),which were stored at room temperature and protected from light for healing. The weight loss and the disease index of wounded tubers inoculated with Fusarium sulphureum were determined, the deposition of suberin polyphenolic and lignin at wounded sites were observed, and the activity of the key enzymes of phenylpropane metabolism and peroxidase as well as the content of phenylpropane metabolism products and H_(2)O_(2) at wounded sites were measured.【Result】The weight loss and the disease index of wounded tubers were significantly reduced by p-CA treatment, which were 38.46% and 43.18% lower than those of control on the 14 day of healing, respectively. p-CA treatment accelerated the deposition of suberin polyphenolic and lignin at wound sites of potato tubers, and the thickness of cell layers in treated tubers were 26.43% and 30.26% higher than that under control on the 14 day of healing, respectively. p-CA treatment also significantly increased the activities of phenylalanine ammonia lyase, cinnamic acid-4-hydroxylase, 4-coumaryl coenzyme A ligase and cinnamyl alcohol dehydrogenase at wounde sites of tubers, with the enzyme activities of the treated tubers being 32.17%, 23.51%, 25.09% and 23.08% higher than the control on 7 day, respectively. p-CA treatment also promoted the synthesis of cinnamic acid, p-coumaric acid, caffeic acid, ferulic acid, sinapic acid and total phenolics, with the contents of cinnamic acid, p-coumaric acid, caffeic acid and total phenolics in the treated tubers on 14 day being 34.26%, 38.29%, 19.31% and 41.04% higher than that under control, respectively, and the contents of ferulic acid, sinapic acid in the treated tubers being 38.33% and 20.47% higher than t

关 键 词：p-CA 马铃薯块茎 愈伤 苯丙烷代谢 

分 类 号：S532[农业科学—作物学]