辣椒胶孢炭疽菌CFEM效应因子鉴定及转录组分析  

Bioinformatic identification and transcriptional analysis of CFEM effectors of Colletotrichum gloeosporioides in pepper

作　　者：刘思珍 欧阳超 满益龙 盛家伟 陈岳[2] 张鑫[2] 郑立敏[2] 李智强 李大伟[6] 张德咏[2] 刘勇[2] 王运生[4] 谭新球 LIU Sizhen;OUYANG Chao;MAN Yilong;SHENG Jiawei;CHEN Yue;ZHANG Xin;ZHENG Limin;LI Zhiqiang;LI Dawei;ZHANG Deyong;LIU Yong;WANG Yunsheng;TAN Xinqiu(Long Ping Branch,Graduate School of Hunan University,Changsha 410125,China;Plant Protection Institute,Hunan Academy ofAgricultural Sciences,Changsha 410125,China;Agricultural Biotechnology Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,China;College of Plant Protection,Hunan Agricultural University,Changsha 410128,China;Plant Protection Institute,Chinese Academy of Agricultural Sciences,Beijing 100193,China;College of Horticulture,Northwest A&F University,Yangling 712100,China)

机构地区：[1]湖南大学研究生院隆平分院,湖南长沙410125 [2]湖南省农业科学院植物保护研究所,湖南长沙410125 [3]湖南省农业科学院生物技术研究所,湖南长沙410125 [4]湖南农业大学植物保护学院,湖南长沙410128 [5]中国农业科学院植物保护研究所,北京100193 [6]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《南京农业大学学报》2021年第6期1074-1082,共9页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(31972223);国家自然科学基金青年基金项目(31701814);湖南农业科技创新资金项目(2020CX39,2020CX43)。

摘　　要：[目的]本文旨在研究辣椒胶孢炭疽菌(Colletotrichum gloeosporioides)常见真菌细胞外膜蛋白(common in several fungal extracellular membrane proteins,CFEM)的组成和结构。[方法]以辣椒胶孢炭疽菌CSLL^(-1)1的基因组为研究对象,通过生物信息学分析其CFEM效应因子的信号肽、跨膜结构域和亚细胞定位信号等结构特征;利用转录组学途径分析其在病菌与寄主互作过程中的表达特征,并对部分CFEM效应因子进行亚细胞定位分析。[结果]CSLL^(-1)1共编码30个CFEM效应因子,其中26个具有信号肽并可分泌至胞外;17个含有数目不等的跨膜结构域。CFEM效应因子Cghn02929、Cghn09727、Cghn12645和Cghn14146在侵染辣椒果实各阶段均具有较高表达水平,推测其可能参与病菌整个侵染过程。Cghn14146、Cghn09727和Cghn13279定位于细胞膜和细胞质,而Cghn02929、Cghn13471定位于细胞核、细胞膜和细胞质。[结论]明确了辣椒胶孢炭疽菌CFEM效应因子的组成和结构特征,探明了CFEM效应因子间的表达水平和亚细胞定位差异。[Objectives]This article aimed to study the composition and structure of several fungal extracellular membrane proteins(CFEM)common in Colletotrichum gloeosporioides which caused pepper anthracnose.[Methods]Taking the genome of pepper anthracnose pathogen CSLL^(-1)1 as the research object,the signal peptide,transmembrane domain and subcellular localization of the CFEM effectors were analyzed by bioinformatics approach.Transcriptomics were used to analyze the expression patterns of CFEM effectors in the stages of infection and analyzed the subcellular location of some CFEM effectors.[Results]The C.gloeosporioides strain CSLL^(-1)1 encoded a total of 30 CFEM effectors,26 of which had signal peptides and were predicted as secreted proteins;17 CFEM effectors contained transmembrane domains.The CFEM effectors Cghn02929,Cghn09727,Cghn12645 and Cghn14146 had high expression levels at all stages of infection,and it was speculated that they might participate in the entire infection and pathogenic process of the pathoge.Cghn14146,Cghn09727 and Cghn13279 were located in the cell membrane and cytoplasm,while Cghn02929 and Cghn13471 were located in the nucleus,cell membrane and cytoplasm.[Conclusions]The composition and structural characteristics of the CFEM effectors of pepper anthracnose were clarified,while the differences both the expression level and subcellular localization of the CFEM effectors were investigated.

关 键 词：辣椒 胶孢炭疽菌 CFEM效应因子 生物信息学分析 亚细胞定位 

分 类 号：S436.418.11[农业科学—农业昆虫与害虫防治]