小麦茎秆强度关联位点及优异等位变异分析  

Analysis of Associated Loci and Elite AllelesAssociated with Stem Strength in Wheat

作　　者：卢杰[1] 田胜明 王胜 陈璨[1] 司红起[1] 常成[1] 马传喜[1] LU Jie;TIAN Shengming;WANG Sheng;CHEN Can;SI Hongqi;CHANG Cheng;MA Chuanxi(School of Agronomy,Anhui Agricultural University/Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow&Huai River Valley,Ministry of Agriculture,Hefei,Anhui 230036,China)

机构地区：[1]安徽农业大学农学院/农业部黄淮南部小麦生物学与遗传育种重点实验室,安徽合肥230036

出　　处：《麦类作物学报》2021年第11期1347-1355,共9页Journal of Triticeae Crops

基　　金：国家现代农业产业技术体系项目(CARS-03);国家重点研发计划项目(SQ2017ZY030040);江苏省现代作物生产协同创新中心资助项目。

摘　　要：为发掘与小麦茎秆强度紧密关联标记位点的优异等位变异和携带优异等位变异的载体资源,本研究以126份小麦种质为材料,基于混合线性模型(mixed linear model,MLM)对2011-2012、2012-2013和2013-2014三个年度的茎秆强度进行标记位点关联分析,并对关联住点等位变异的表型效应进行分析。结果表明,wmc83(2B)、gwm539(2D)、barc358(5A)、barc59(5B)和barc134(6B)均与茎秆强度显著关联,且可在3个环境下同时检测到,表型解释率均大于8%;共发掘出11种优异等位变异,分别为wmc83-A110、wmc83-A147、wmc83-A151、gwm539-A120、barc358-A179/161、barc358-A185/161、barc358-A185/179、barc358-A190/161、barc59-A182、barc59-A191和barc134-A194,其中wmc83-A110的增效效应最大。供试材料的茎秆强度随优异等位变异聚合数目的增多而增大,其中黄淮南片麦区、黄淮北片麦区、长江中下游麦区和西南麦区的供试材料中携带2(40.3%)、1(27.8%)、1(35.3%)和4(50.0%)种优异等位变异的分布频率最高。内麦10号等9份材料聚合4种及以上的优异等位变异,且茎秆强度较高,可作为相应麦区小麦茎秆强度遗传改良的种质资源。In order to explore the elite alleles associated with stem strength in wheat and materials carrying these elite alleles,in this study,126 wheat materials were analyzed by using mixed line model(MLM)in three year,and the allelic effects of the associated loci were analyzed.The results showed that five stable loci(wmc83,gwm539,barc358,barc59 and barc134)distributed on 2B,2D,5A,5B and 6B chromosomes were detected in three year,and the phenotypic interpretation rate was more than 8%.A total of 11 elite alleles were further explored at the three stable loci.They are wmc83-A110,wmc83-A147,wmc83-A151,gwm539-A120,barc358-A179/161,barc358-A185/161,barc358-A185/179,barc358-A190/161,barc59-A182,barc59-A191 and barc134-A194,among which wmc83-A110 have a strongest allelic effect on increasing stem strength.The stem strength of the materials increased with the number of the elite allelic variations.The distribution frequency of materials in South Yellow-Huai River Valleys Wheat Region,North Yellow-Huai River Valleys Wheat Region,Middle and Low Yangtze Valleys Wheat Region and Southwest Wheat Region corrying 2(40.3%),1(27.8%),1(35.3%)and 4(50.0%)elite alleles are highest,respectively.Nine materials(e.g.Neimai 10)containing more than 4 elite alleles can be used as important resources for improving wheat stem strength in corresponding wheat regions.

关 键 词：小麦 茎秆强度 分子标记 关联分析 等位变异 

分 类 号：S512.1[农业科学—作物学] S330