红条毛肤石鳖和日本花棘石鳖的转录组比较分析  

Comparative Transcriptome Analysis of Acanthochitona rubrolineata and Liolophuura japonica

作　　者：张倩 吴一晶 ZHANG Qian;WU Yijing(Institute of Oceanography, Minjiang University, Fuzhou, Fujian 350108, China)

机构地区：[1]闽江学院海洋研究院,福建福州350108

出　　处：《闽江学院学报》2021年第5期92-101,共10页Journal of Minjiang University

基　　金：福建省教育厅中青年教师教育科研项目(JT180409)。

摘　　要：多板纲动物具有独特的形态结构,而且在软体动物的系统进化中占有重要地位,但目前有关多板纲的分子生物学研究十分欠缺。该研究通过RNA-seq技术对红条毛肤石鳖和日本花棘石鳖进行了转录组测序,组装后分别获得121892和83776个Unigene,虽然两个物种属于不同的科,但GO和KEGG功能富集分析结果显示二者表达基因较为一致,SSR在转录本中的数量与分布也无显著差异。此外,鉴于Pax基因与动物形态结构发育密切相关,且在多板纲中的研究几乎为空白,所以该研究着重关注了Pax基因。通过转录组数据分析,共筛选到8条Pax基因,在系统进化树中均与相对应的软体动物Pax基因亚族聚为一支,表明多板纲的Pax基因进化保守。该研究获得的转录组信息丰富了多板纲的分子数据,为进一步探究多板纲动物生物学性状提供了良好的遗传信息基础。Polyplacophora with unique morphological structure plays an important role in the systemic evolution of molluscs.However,there is a shortage of systematical molecular study on Polyplacophora.In this study,RNA-seq technology was used to sequence the transcriptome of Acanthochitona rubrolineata(A.rubrolineata)and Liolophuura japonica(L.japonica).Results showed that there were 121892 and 83776 unigenes in each transcriptome,respectively.Although the two species belong to different families,the results of GO and KEGG functional enrichment analysis showed that the expressed genes were relatively consistent,and the number and distribution of SSR in transcriptome of A.rubrolineata and L.japonica were not significantly different.Furthermore,the study focused on Pax gene which was closely related to the development of morphology but the functions still remained unclear.Eight Pax genes were screened in two transcriptomes,all of which clustered with the corresponding Pax gene subfamily of mollusks in the phylogenetic tree,showing that the Pax genes of the Polyplacophora were conserved.The transcriptome information obtained enriched the molecular data of Polyplacophora and could provide a basis and reference for further exploration.

关 键 词：红条毛肤石鳖 日本花棘石鳖 比较转录组 Pax基因家族 

分 类 号：Q75[生物学—分子生物学]