Incorporating redox-sensitive nanogels into bioabsorbable nanofibrous membrane to acquire ROS-balance capacity for skin regeneration  

作　　者：Shihao Zhang Yamin Li Xiaofeng Qiu Anqi Jiao Wei Luo Xiajie Lin Xiaohui Zhang Zeren Zhang Jiachan Hong Peihao Cai Yuhong Zhang Yan Wu Jie Gao Changsheng Liu Yulin Li 

机构地区：[1]The Key Laboratory for Ultrafine Materials of Ministry of Education,State Key Laboratory of Bioreactor Engineering,Engineering Research Center for Biomedical Materials of Ministry of Education,School of Materials Science and Engineering,East China University of Science and Technology,Shanghai,200237,China [2]Institute of Translational Medicine,Shanghai University,Shanghai,200444,China [3]Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy,Mudanjiang Medical University,Mudanjiang,157011,China [4]Shanghai Jiaotong University Affiliated Sixth People’s Hospital,Shanghai,200233,China [5]Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials,Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules,College of Chemistry and Chemical Engineering,Hubei University,Wuhan,430062,China

出　　处：《Bioactive Materials》2021年第10期3461-3472,共12页生物活性材料（英文）

基　　金：The research was supported by National Key R&D Program of China(2018YFE0201500,2017YFB0309300);National Natural Science Foundation of China(81772317,51973060,82072051,81771964);National Natural Science Foundation of China for Innovative Research Groups(51621002);Natural Science Foundation of Heilongjiang Province(LH2020H076);Shanghai International Cooperation Program(15520721200);Central Universities(WD1714002);The funding grants from Joint research project of important diseases in Xuhui District(XHLHGG201802);Fundamental Research Funds for the Shanghai Municipal Commission of Health and Family Planning(201740034)were also acknowledged.

摘　　要：Facing the high incidence of skin diseases,it is urgent to develop functional materials with high bioactivity for wound healing,where reactive oxygen species(ROS)play an important role in the wound healing process mainly via adjustment of immune response and neovasculation.In this study,we developed a kind of bioabsorbable materials with ROS-mediation capacity for skin disease therapy.Firstly,redox-sensitive poly(N-isopropylacrylamide-acrylic acid)(PNA)nanogels were synthesized by radical emulsion polymerization method using a disulfide molecule as crosslinker.The resulting nanogels were then incorporated into the nanofibrous membrane of poly(L-lactic acid)(PLLA)via airbrushing approach to offer bioabsorbable membrane with redox-sensitive ROS-balance capacity.In vitro biological evaluation indicated that the PNA-contained bioabsorbable membrane improved cell adhesion and proliferation compared to the native PLLA membrane.In vivo study using mouse wound skin model demonstrated that PNA-doped nanofibrous membranes could promote the wound healing process,where the disulfide bonds in them were able to adjust the ROS level in the wound skin for mediation of redox potential to achieve higher wound healing efficacy.

关 键 词：POLYLACTIDE Nanofibrous membrane Redox sensitivity ROS-Balance capacity Skin regeneration 

分 类 号：R318.08[医药卫生—生物医学工程]