Bioactive glass promotes the barrier functional behaviors of keratinocytes and improves the Re-epithelialization in wound healing in diabetic rats  

作　　者：Fengling Tang Junliang Li Weihan Xie Yunfei Mo Lu Ouyang Fujian Zhao Xiaoling Fu Xiaofeng Chen 

机构地区：[1]Department of Biomedical Engineering,School of Materials Science and Engineering,South China University of Technology,Guangzhou 510641,PR China [2]National Engineering Research Center for Tissue Restoration and Reconstruction,Guangzhou 510006,PR China [3]Key Laboratory of Biomedical Engineering of Guangdong Province,and Innovation Center for Tissue Restoration and Reconstruction,South China University of Technology,Guangzhou 510006,PR China [4]Stomatological Hospital,Southern Medical University,Guangzhou 510280,PR China

出　　处：《Bioactive Materials》2021年第10期3496-3506,共11页生物活性材料（英文）

基　　金：This work was financially supported by the National Key R&D Program of China(2017YFC1105000);the National Natural Science Foundation of China(51672088,31971266,U1801252);the Key Research and Development Program of Guangzhou(202007020002);the Natural Science Foundation of Guangdong Province(2019A1515110480);the Science and Technology Program of Guangzhou(201804020060);Fundamental Research Funds for the Central Universities.

摘　　要：Upon skin injury,re-epithelialization must be triggered promptly to restore the integrity and barrier function of the epidermis.However,this process is often delayed or interrupted in chronic wounds like diabetic foot ulcers.Considering that BG particles can activate multiple genes in various cells,herein,we hypothesized that bioactive glass(BG)might be able to modulate the barrier functional behaviors of keratinocytes.By measuring the transepithelial electrical resistance(TEER)and the paracellular tracer flux,we found the 58S-BG extracts substantially enhanced the barrier function of keratinocyte monolayers.The BG extracts might exert such effects by promoting the keratinocyte differentiation and the formation of tight junctions,as evidenced by the increased expression of critical differentiation markers(K10 and involucrin)and TJ protein claudin-1,as well as the altered subcellular location of four major TJ proteins(claudin-1,occludin,JAM-A,and ZO-1).Besides,the cell scratch assay showed that BG extracts induced the collective migration of keratinocytes,though they did not accelerate the migration rate compared to the control.The in vivo study using a diabetic rat wound model demonstrated that the BG extracts accelerated the process of re-epithelialization,stimulated keratinocyte differentiation,and promoted the formation of tight junctions in the newly regenerated epidermis.Our findings revealed the crucial effects of BGs on keratinocytes and highlighted its potential application for chronic wound healing by restoring the barrier function of the wounded skin effectively.

关 键 词：Bioactive glass KERATINOCYTE Barrier function Diabetic wound healing 

分 类 号：R318.08[医药卫生—生物医学工程]