A novel dentin bonding scheme based on extrafibrillar demineralization combined with covalent adhesion using a dry-bonding technique  

作　　者：F.Yu M.L.Luo R.C.Xu L.Huang H.H.Yu M.Meng J.Q.Jia Z.H.Hu W.Z.Wu F.R.Tay Y.H.Xiao L.N.Niu J.H.Chen 

机构地区：[1]Department of Stomatology,920 Hospital of Joint Logistics Support Force,PLA,Kunming,650032,China [2]Department of Stomatology,The First Medical Center,Chinese PLA General Hospital,Beijing,China [3]Department of Stomatology,The Third Medical Center,Chinese PLA General Hospital,Beijing,China [4]Department of General Dentistry and Emergency,School of Stomatology,The Fourth Military Medical University,Xi’an,China [5]National Clinical Research Center for Oral Diseases&Shaanxi Key Laboratory of Stomatology,Department of Prosthodontics,School of Stomatology,The Fourth Military Medical University,Xi’an,China [6]The Dental College of Georgia,Augusta University,Augusta,GA,30912,USA

出　　处：《Bioactive Materials》2021年第10期3557-3567,共11页生物活性材料（英文）

基　　金：This work was supported by National Natural Science Foundation of China(81720108011,81801009,81460107,81970972 and 82001110);the program for Changjiang Scholars and Innovative Research Team in University(No.IRT13051).

摘　　要：Dentin bonding is a dynamic process that involves the penetration of adhesive resin monomers into the extrafibrillar and intrafibrillar demineralized collagen matrix using a wet-bonding technique.However,adhesive resin monomers lack the capacity to infiltrate the intrafibrillar space,and the excess water that is introduced by the wet-bonding technique remains at the bonding interface.This imperfectly bonded interface is inclined to hydrolytic degradation,severely jeopardizing the longevity of bonded clinical restorations.The present study introduces a dentin bonding scheme based on a dry-bonding technique,combined with the use of extrafibrillar demineralization and a collagen-reactive monomer(CRM)-based adhesive(CBA).Selective extrafibrillar demineralization was achieved using 1-wt%high-molecular weight(MW)carboxymethyl chitosan(CMCS)within a clinically acceptable timeframe to create a less aggressive bonding substance for dentin bonding due to its selectively extrafibrillar demineralization capacity.CMCS demineralization decreased the activation of in situ collagenase,improved the shrinking resistance of demineralized collagen,and thus provided stronger and more durable bonding than traditional phosphoric acid etching.The new dentin bonding scheme that contained CMCS and CBA and used a dry-bonding technique achieved an encouraging dentin bonding strength and durability with low technical sensitivity.This bonding scheme can be used to improve the stability of the resin-dentin interface and foster the longevity of bonded clinical restorations.

关 键 词：DENTIN ADHESIVE COLLAGEN Carboxymethyl chitosan Extrafibrillar 

分 类 号：R318.08[医药卫生—生物医学工程]