Biomimetic injectable hydrogel microspheres with enhanced lubrication and controllable drug release for the treatment of osteoarthritis  

作　　者：Ying Han Jielai Yang Weiwei Zhao Haimang Wang Yulong Sun Yuji Chen Jing Luo Lianfu Deng Xiangyang Xu Wenguo Cui Hongyu Zhang 

机构地区：[1]State Key Laboratory of Tribology,Department of Mechanical Engineering,Tsinghua University,Beijing,100084,China [2]Department of Orthopaedics,Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases,Shanghai Institute of Traumatology and Orthopaedics,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200025,China [3]Beijing Research Institute of Automation for Machinery Industry Co.,Ltd,Beijing,100120,China

出　　处：《Bioactive Materials》2021年第10期3596-3607,共12页生物活性材料（英文）

基　　金：This study was financially supported by National Natural Science Foundation of China(52022043 and 81930051);Tsinghua University-Peking Union Medical College Hospital Initiative Scientific Research Program(20191080593);Precision Medicine Foundation,Tsinghua University,China(10001020107);Shanghai Jiao Tong University“Medical and Research”Program(ZH2018ZDA04);Science and Technology Commission of Shanghai Municipality(18ZR1434200,18140901500 and 19440760400);Research Fund of State Key Laboratory of Tribology,Tsinghua University,China(SKLT2020C11).

摘　　要：The occurrence of osteoarthritis(OA)is highly associated with the reduced lubrication property of the joint,where a progressive and irreversible damage of the articular cartilage and consecutive inflammatory response dominate the mechanism.In this study,bioinspired by the super-lubrication property of cartilage and catecholamine chemistry of mussel,we successfully developed injectable hydrogel microspheres with enhanced lubrication and controllable drug release for OA treatment.Particularly,the lubricating microspheres(GelMA@DMA-MPC)were fabricated by dip coating a self-adhesive polymer(DMA-MPC,synthesized by free radical copolymerization)on superficial surface of photo-crosslinked methacrylate gelatin hydrogel microspheres(GelMA,prepared via microfluidic technology),and encapsulated with an anti-inflammatory drug of diclofenac sodium(DS)to achieve the dual-functional performance.The tribological test and drug release test showed the enhanced lubrication and sustained drug release of the GelMA@DMA-MPC microspheres.In addition,the functionalized microspheres were intra-articularly injected into the rat knee joint with an OA model,and the biological tests including qRT-PCR,immunofluorescence staining assay,X-ray radiography and histological staining assay all revealed that the biocompatible microspheres provided significant therapeutic effect against the development of OA.In summary,the injectable hydrogel microspheres developed herein greatly improved lubrication and achieved sustained local drug release,therefore representing a facile and promising technique for the treatment of OA.

关 键 词：Microfluidics Hydrogel microspheres Catecholamine chemistry Hydration lubrication Drug delivery 

分 类 号：R318.08[医药卫生—生物医学工程] R68[医药卫生—基础医学]