Orchestrated cellular,biochemical,and biomechanical optimizations endow platelet-rich plasma-based engineered cartilage with structural and biomechanical recovery  

作　　者：Ketao Wang Ji Li Yuxing Wang Yaqiang Wang Yuanyuan Qin Fei Yang Mingzhu Zhang Heng Zhu Zhongli Li 

机构地区：[1]Department of Orthopedics,Chinese PLA General Hospital,Haidian,Beijing,100853,China [2]Beijing Institute of Radiation Medicine/Beijing Institute of Basic Medical Sciences,Haidian,Beijing,100850,China [3]Department of Foot and Ankle,Beijing Tongren Hospital,Capital Medical University,Beijing,100730,China [4]Beijing National Laboratory for Molecular Sciences,Institute of Chemistry,Chinese Academy of Sciences,Beijing,100190,China [5]Department of Orthopedic Surgery,Zhongshan Hospital,Fudan University,Shanghai,200032,China [6]Department of Blood Transfusion,Chinese PLA General Hospital,Haidian,Beijing,100853,China

出　　处：《Bioactive Materials》2021年第11期3824-3838,共15页生物活性材料（英文）

基　　金：the National Natural Science Foundation of China(81871771,81572159);the Beijing Natural Sciences Grants(7182123,7191010).

摘　　要：Recently,biomaterials for cartilage regeneration has been intensively investigated.However,the development of scaffolds that capture regenerated cartilage with biomechanical and structural recovery has rarely been reported.To address this challenge,platelet-rich plasma(PRP)-based cartilage constructs with a well-orchestrated symphony of cellular,biochemical and biomechanical elements were prepared by simultaneously employing chondrogenic progenitor cells(CPCs)as a cell source,optimizing platelet concentration,and adding an enzyme-ion activator.It was shown that this triple-optimized PRP+CPC construct possessed increased biomechanical properties and suitable biochemical signals.The following in vitro study demonstrated that the triple-optimized PRP+CPC constructs generated cartilage-like tissue with higher expression levels of chondrogenic-specific markers,more deposition of cartilage-specific extracellular matrix(ECM),and greater biomechanical values than those of the other constructs.Twelve weeks after the construct was implanted in a cartilage defect in vivo,histological analysis,qPCR,and biomechanical tests collectively showed that the triple-optimized constructs yielded a more chondrocyte-like cell phenotype with a higher synthesis of Col-Ⅱand aggrecan.More importantly,the triple-optimized constructs facilitated cartilage regeneration with better biomechanical recovery than that of the other constructs.These results demonstrate the efficacy of the triple-optimization strategy and highlight the simplicity and potency of this PRP+CPC construct for cartilage regeneration.

关 键 词：Platelet-rich plasma Cartilage regeneration Structural recovery Biomechanical recovery 

分 类 号：R318[医药卫生—生物医学工程]