Endometrial membrane organoids from human embryonic stem cell combined with the 3D Matrigel for endometrium regeneration in asherman syndrome  

作　　者：Xiuxiu Jiang Xingmiao Li Xiangwei Fei Jiajie Shen Jianhua Chen Meijun Guo Yangyang Li 

机构地区：[1]Department of Gynecology,Women’s Hospital,Zhejiang University School of Medicine,Hangzhou City,Zhejiang Province,310006,China [2]Key Laboratory of Women′s Reproductive Health Research of Zhejiang Province,Women’s Hospital,Zhejiang University School of Medicine,Hangzhou City,Zhejiang Province,310006,China [3]Department of Pathology,Women’s Hospital,Zhejiang University School of Medicine,Hangzhou City,Zhejiang Province,310006,China

出　　处：《Bioactive Materials》2021年第11期3935-3946,共12页生物活性材料（英文）

基　　金：the National Nature Science Foundation of China(grant no.81401179).

摘　　要：Asherman’s syndrome(AS),a leading cause of uterine infertility worldwide,is characterized by scarring of the uterine surfaces lacking endometrial epithelial cells,which prevents endometrial regeneration.Current research on cell therapy for AS focuses on mesenchymal and adult stem cells from the endometrium.However,insufficient number,lack of purity,and rapid senescence of endometrial epithelial progenitor cells(EEPCs)during experimental processes restrict their use in cell therapies.In this study,we induced human embryonic stem cells-9(H9-ESC)into EEPCs by optimizing the induction factors from the definitive endoderm.EEPCs,which act as endometrial epithelial cells,accompanied by human endometrial stromal cells provide a niche environment for the development of endometrial membrane organoids(EMOs)in an in vitro 3D culture model.To investigate the function of EMOs,we transplanted tissue-engineered constructs with EMOs into an in vivo rat AS model.The implantation of EMOs into the damaged endometrium facilitates endometrial regeneration and angiogenesis.Implanting EMOs developed from human embryonic stem cells into the endometrium might prove useful for“endometrial re-engineering”in the treatment of Asherman’s syndrome.

关 键 词：Asherman’s syndrome H9-ESC Rat AS model Endometrial membrane organoids 3D culture 

分 类 号：R711.74[医药卫生—妇产科学] R318[医药卫生—临床医学]