Sand-mediated ice seeding enables serum-free low-cryoprotectant cryopreservation of human induced pluripotent stem cells  

作　　者：Bin Jiang Weijie Li Samantha Stewart Wenquan Ou Baolin Liu Pierre Comizzoli Xiaoming He 

机构地区：[1]Fischell Department of Bioengineering,University of Maryland,College Park,MD,20742,USA [2]Institute of Biothermal Technology,University of Shanghai for Science and Technology,Shanghai,200093,China [3]Smithsonian Conservation Biology Institute,National Zoological Park,Washington,DC,20008,USA [4]Robert E.Fischell Institute for Biomedical Devices,University of Maryland,College Park,MD,20742,USA [5]Marlene and Stewart Greenebaum Comprehensive Cancer Center,University of Maryland,Baltimore,MD,21201,USA

出　　处：《Bioactive Materials》2021年第12期4377-4388,共12页生物活性材料（英文）

基　　金：This work was partially supported by grants from the US National Science Foundation(CBET-1831019 to XH and DGE-1840340 that is a GRFP fellowship to SS);National Institutes of Health(NIH R01EB023632 to XH).

摘　　要：Human induced pluripotent stem cells(hiPSCs)possess tremendous potential for tissue regeneration and banking hiPSCs by cryopreservation for their ready availability is crucial to their widespread use.However,contemporary methods for hiPSC cryopreservation are associated with both limited cell survival and high concentration of toxic cryoprotectants and/or serum.The latter may cause spontaneous differentiation and/or introduce xenogeneic factors,which may compromise the quality of hiPSCs.Here,sand from nature is discovered to be capable of seeding ice above10◦C,which enables cryopreservation of hiPSCs with no serum,much-reduced cryoprotectant,and high cell survival.Furthermore,the cryopreserved hiPSCs retain high pluripotency and functions judged by their pluripotency marker expression,cell cycle analysis,and capability of differentiation into the three germ layers.This unique sand-mediated cryopreservation method may greatly facilitate the convenient and ready availability of high-quality hiPSCs and probably many other types of cells/tissues for the emerging cell-based translational medicine.

关 键 词：SAND Ice seeding CRYOPRESERVATION iPSC Stem cell 

分 类 号：R318[医药卫生—生物医学工程]