Durable endothelium-mimicking coating for surface bioengineering cardiovascular stents  

作　　者：Qing Ma Xiuying Shi Xing Tan Rui Wang Kaiqin Xiong Manfred F.Maitz Yuanyuan Cui Zhangmei Hu Qiufen Tu Nan Huang Li Shen Zhilu Yang 

机构地区：[1]Key Lab of Advanced Technology of Materials of Education Ministry,School of Materials Science and Engineering,Yibin Institute of Southwest Jiaotong University,Southwest Jiaotong University,Chengdu,610031,China [2]Shanghai Institute of Cardiovascular Diseases,Department of Cardiology,Zhongshan Hospital,Fudan University,180 Fenglin Road,Shanghai,200032,China [3]Max Bergmann Center of Biomaterials,Leibniz Institute of Polymer Research Dresden,Hohe Strasse 6,01069,Dresden,Germany [4]Shimazu China Co.LTD.,No.180 Yizhou Road,Xuhui District,Shanghai,200233,China [5]Analysis&Testing Center,Southwest Jiaotong University,Chengdu,610031,China

出　　处：《Bioactive Materials》2021年第12期4786-4800,共15页生物活性材料（英文）

基　　金：This work was supported by the National Natural Science Foundation of China(Project 82072072);International Cooperation Project by Science and Technology Department of Sichuan Province(2021YFH0056,2019YFH0103);the Fundamental Research Funds for the Central Universities(2682020ZT82,2682020ZT76).

摘　　要：Mimicking the nitric oxide(NO)-release and glycocalyx functions of native vascular endothelium on cardiovascular stent surfaces has been demonstrated to reduce in-stent restenosis(ISR)effectively.However,the practical performance of such an endothelium-mimicking surfaces is strictly limited by the durability of both NO release and bioactivity of the glycocalyx component.Herein,we present a mussel-inspired amine-bearing adhesive coating able to firmly tether the NO-generating species(e.g.,Cu-DOTA coordination complex)and glycocalyx-like component(e.g.,heparin)to create a durable endothelium-mimicking surface.The stent surface was firstly coated with polydopamine(pDA),followed by a surface chemical cross-link with polyamine(pAM)to form a durable pAMDA coating.Using a stepwise grafting strategy,Cu-DOTA and heparin were covalently grafted on the pAMDA-coated stent based on carbodiimide chemistry.Owing to both the high chemical stability of the pAMDA coating and covalent immobilization manner of the molecules,this proposed strategy could provide 62.4%bioactivity retention ratio of heparin,meanwhile persistently generate NO at physiological level from 5.9±0.3 to 4.8±0.4×10^(-10) mol cm^(-2) min^(-1) in 1 month.As a result,the functionalized vascular stent showed long-term endothelium-mimicking physiological effects on inhibition of thrombosis,inflammation,and intimal hyperplasia,enhanced re-endothelialization,and hence efficiently reduced ISR.

关 键 词：Cardiovascular stents Nitric oxide Glycocalyx component Endothelium mimicking Surface bioengineering 

分 类 号：R318.08[医药卫生—生物医学工程]