Metabolomics analysis of poly(L-lactic acid)nanofibers’performance on PC12 cell differentiation  

作　　者：Xiaoman Su Yan Huang Rong Chen Yiwen Zhang Meichen He Xiaoying Lu 

机构地区：[1]State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,2#Si Pailou,Nanjing 210096,China [2]Department of Research,SQ Medical Device Co.,Ltd,17#Xinghuo Road,Nanjing 211500,China [3]Coinnovation Center of Neuroregeneration,Nantong University,Nantong,Jiangsu Province 226019,China

出　　处：《Regenerative Biomaterials》2021年第4期72-87,共16页再生生物材料（英文版）

基　　金：This study received the support of the National Natural Science Foundation of China(31170910,31971254 and 31271012).

摘　　要：The aim of this article is to reveal the influence of aligned/random poly(L-lactic acid)(PLLA)nanofibers on PC12 cell differentiation from the perspective of metabolic level.First,three materials-PLLA aligned nanofibers(PLLA AF),PLLA random nanofibers(PLLA RF)and PLLA films(control)-were prepared by electrospinning and spin coating.Their surface morphologies were characterized.Subsequently,the cell viability,cell morphology and neurite length of PC12 cells on the surface of the three materials were evaluated,indicating more neurites in the PLLA RF groups but the longer average neurite length in the PLLA AF groups.Next,the metabolite profiles of PC12 cells cultured on the surface of the three nanofibers after 12 h,24 h and 36 h showed that,compared with the control,51,48 and 31 types of differential metabolites were detected at the three time points among the AF groups,respectively;and 56,45 and 41 types among the RF groups,respectively.Furthermore,the bioinformatics analysis of differential metabolites identified two pathways and three metabolites critical to PC12 cell differentiation influenced by the nanofibers.In addition,the verification experiment on critical metabolites and metabolic pathways were performed.The integrative analysis combining cytology,metabolomics and bioinformatics approaches revealed that though both PLLA AF and RF were capable of stimulating the synthesis of neurotransmitters,the PLLA AF were more beneficial for PC12 cell differentiation,whereas the PLLA RF were less effective.

关 键 词：NANOFIBERS metabolomics and pathway analysis PC12 cell differentiation molecular mechanism 

分 类 号：R318[医药卫生—生物医学工程]