ROS-responsive biomimetic nanoparticles for potential application in targeted anti-atherosclerosis  

作　　者：Dan Tang Yi Wang Andy Wijaya Boyan Liu Ali Maruf Jinxuan Wang Jianxiong Xu Xiaoling Liao Wei Wu Guixue Wang 

机构地区：[1]Key Laboratory for Biorheological Science and Technology of Ministry of Education,State and Local Joint Engineering Laboratory for Vascular Implants,Bioengineering College of Chongqing University,Chongqing 400030,China [2]Chongqing Key Laboratory of Nano/Micro Composite Material and Device,School of Metallurgy and Materials Engineering,Chongqing University of Science and Technology,Chongqing 401331,China

出　　处：《Regenerative Biomaterials》2021年第4期97-106,共10页再生生物材料（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(31971301,31971242,12032007);the China Postdoctoral Science Foundation(2020M673143);the Natural Science Foundation of Chongqing(cstc2020jcyj-bsh0025,cstc2019jcyj-zdxmX0028,cstc2017jcyjAX0186);Fundamental Research Funds for Central Universities(2020CDJQY-A061,2020CDJYGSX006,2019CDYGZD008,2018CDHB1B08)as well as the National‘111 Project’Base(B0625)are gratefully acknowledged.

摘　　要：The development of nanomedicines provides new opportunities for the treatment of atherosclerosis(AS)due to their great advantages such as the improved drug solubility,enhanced bioavailability and reduced side effects.Despite these advantages,nanomedicines are still facing some challenges.The problems remain in the short circulation life,lack of specific targeting and poor drug release controllability.In order to overcome the shortages of conventional nanomedicines,the combination of biomimetic strategy with smart nanoagents has been proposed.In light with the high reactive oxygen species(ROS)level in AS microenvironment and the fact that macrophages play a critical role in the pathogenesis of AS,we fabricated ROS-responsive biomimetic nanoparticles(NPs),which camouflaged macrophage membrane(MM)on ROS-responsive NPs loaded with rapamycin(RNPs)for potential application in AS therapy.The resulting ROSresponsive biomimetic NPs(MM/RNPs)exhibited favorable hydrodynamic size with negative surface charge,retained the functional proteins from MM,and showed ROS-responsive drug release.Because of the biomimetic camouflaging on surface,MM/RNPs could effectively escape from macrophages uptake and target to inflammatory endothelial cells.Meanwhile,MM/RNPs could inhibit the proliferation of macrophages and smooth muscle cells in vitro.Furthermore,the MM-coated NPs were found to be nontoxic in both cytotoxicity assay and in vivo toxicity evaluation.Consequently,these results demonstrated that MM/RNPs could be a potential candidate of drug delivery system for safe and effective anti-AS applications.

关 键 词：biomimetic nanoparticles macrophage membrane ROS-responsive targeted delivery atherosclerosis 

分 类 号：R543.5[医药卫生—心血管疾病] R318.08[医药卫生—内科学]