产黄青霉Penicillium chrysogenum S-3-25的次级代谢产物及其活性研究  

The secondary metabolites isolated from Penicillium chrysogenum S-3-25 and their bioactivities

作　　者：聂丹 杨郁 赵雪 陈波[2] 朱天骄[3] 单俊杰 李长伟 崔承彬 NIE Dan;YANG Yu;ZHAO Xue;CHEN Bo;ZHU Tian-jiao;SHAN Jun-jie;LI Chang-wei;CUI Cheng-bin(Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China;Key Laboratory for Polar Science of State Oceanic Administration,Polar Research Institute of China,Shanghai 200136,China;Key Laboratory of Marine Drugs,Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China)

机构地区：[1]军事医学研究院毒物药物研究所,北京100850 [2]中国极地研究中心国家海洋局极地科学重点实验室,上海200136 [3]中国海洋大学海洋药物教育部重点实验室医药学院,山东青岛266003

出　　处：《中国海洋药物》2021年第5期1-8,共8页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(81573300)资助。

摘　　要：目的阐明南极深海来源真菌产黄青霉Penicillium chrysogenum S-3-25的次级代谢产物及其活性。方法利用各种色谱技术分离纯化次级代谢产物,根据理化和波谱数据(核磁共振、质谱和单晶衍射技术)鉴定化合物结构,采用3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)法评价细胞毒活性。结果从真菌S-3-25的大米发酵产物中分离鉴定了7个单体化合物:meleagrin(1),cyclo(dehydrohistidyl-L-tryptophyl)(2),sohirnone A(3),dihydrodemethylsorbicillin(4),2’,3’-dihydrosorbicillin(5),脑苷脂A(6)及脑苷脂B(7)。其中化合物1对小鼠小胶质BV2细胞和人乳腺癌MCF-7细胞的IC_(50)分别为0.9、20.3μmol·L^(-1)。结论从极地深海来源真菌产黄青霉S-3-25中分离得到7个单体化合物,其中化合物2、4、6、7未见从该菌分离的报道。化合物1对BV2、MCF-7细胞显示出中等细胞毒活性。Objective To investigate the bioactive secondary metabolites of a fungus Penicillium chrysogenum S-3-25 isolated from polar deep sea.Methods The secondary metabolites were isolated by multiple separation techniques,and the structures of the compounds were identified according to the physicochemical and spectral data(nuclear magnetic resonance,mass spectrometry and single crystal X-ray diffraction),and the cytotoxic activities were assayed by MTT method.Results Seven metabolites:meleagrin(1),cyclo(dehydrohistidyl-L-tryptophyl)(2),sohirnone A(3),dihydrodemethylsorbicillin(4),2’,3’-dihydrosorbicillin(5),cerebroside A(6)and cerebroside B(7)were isolated and identified from its rice fermentation products.The IC_(50) of 1 against BV2 and MCF-7 cells were 0.9 and 20.3μmol·L^(-1),respectively.Conclusion Seven compounds were isolated from the fungus Penicillium chrysogenum S-3-25,and 2,4,6 and 7 were first reported to be isolated from P.chrysogenum.Compound 1 showed moderate cytotoxic activities on BV2 and MCF-7 cells.

关 键 词：海洋来源真菌 产黄青霉 代谢产物 细胞毒 

分 类 号：R931.77[医药卫生—生药学]