Investigation of the metabolites of five major constituents from Berberis amurensis in normal and pseudo germ-free rats  

作　　者：LIAO Cui-Ping LIU Xing-Chao DONG Shi-Qi AN Ming ZHAO Lu ZHANG Ai-Jie LIU Jian-Feng HOU Wen-Bin FAN Hui-Rong LIU Chang-Xiao 

机构地区：[1]Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine,Institute of Radiation Medicine,Chinese Academy of Medical Sciences&Peking Union Medical College,Tianjin 300000,China [2]Medical experimental center,China Academy of Chinese Medical Sciences,Beijing 100700,China [3]College of pharmacy,Hebei University of Chinese Medicine,Shijiazhuang 050200,China [4]College of pharmacy,Baotou Medical College,Baotou 014000,China [5]College of pharmacy,Tianjin University of Traditional Chinese Medicine,Tianjin 300000,China [6]State Key Laboratory of Drug Delivery Technology and Pharmacokinetics,Tianjin Institute of Pharmaceutical Research,Tianjin 300000,China

出　　处：《Chinese Journal of Natural Medicines》2021年第10期758-771,共14页中国天然药物（英文版）

基　　金：supported by the National Key R&D Program of China(No.2018YFC1708203);the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences(No.2018PT35031);the Drug Innovation Major Project(No.2018ZX09711001-002-002);the Major Science and Technology Project of Inner Mongolia Autonomous Region(No.2019ZD004).

摘　　要：Berberis amurensis(Berberidaceae)is a traditional Chinese medicine,which is often used to treat hypertension,inflammation,dysentery and enteritis.It contains alkaloids,mainly including berberine,berbamine,magnoflorine,jatrorrhizine and palmatine.Berberis amurensis extracts(BAEs)is often orally taken.Oral herbs might be metabolized by intestinal bacteria in the small intestine.However,the interaction between the herb and the gut microbiota is still unknown.In the current study,UPLC/Q-TOFMS/MS combined with Metabolitepilot and Peakview software was used to identify the metabolites of BAEs in anti-biotic cocktail induced pseudo germ-free rats and normal rats.As a result,a total of 46 metabolites in normal rats were detected and its main metabolic pathways include demethylation,dehydrogenation,methylation,hydroxylation,sulfation and glucuronidation.Only 29 metabolites existed in pseudo germ-free rats.Dehydrogenated metabolites(M29,M30,M34 and M36),methylated metabolites(M33,M41 and M46)and other metabolites were not detected in pseudo germ-free rats.The result implied that the intestinal bacteria have an influence on the metabolism of BAEs.Furthermore,this investigation might contribute to the understanding of the metabolism of BAEs,and further promote its clinical application.

关 键 词：Berberis amurensis BERBERIDACEAE Pseudo germ-free rat Intestinal flora UPLC/Q-TOF-MS/MS 

分 类 号：R944[医药卫生—药剂学]