滤泡辅助性T淋巴细胞与儿童嗜酸性粒细胞性胃肠炎的相关性研究  

Correlation between T follicular helper cells and eosinophilic gastroenteritis in children

作　　者：郭红梅[1] 范进霞 张兰芳 刘志峰[1] Guo Hongmei;Fan Jinxia;Zhang Lanfang;Liu Zhifeng(Department of Gastroenterology,Children′s Hospital of Nanjing Medical University,Nanjing 210008,China)

机构地区：[1]南京医科大学附属儿童医院消化科,210008

出　　处：《中华实用儿科临床杂志》2021年第19期1480-1483,共4页Chinese Journal of Applied Clinical Pediatrics

摘　　要：目的探讨滤泡辅助性T淋巴(Tfh)细胞在儿童嗜酸性粒细胞性胃肠炎(EG)发病中的作用及临床意义。方法选取2018年10月至2020年1月在南京医科大学附属儿童医院消化科住院的EG患儿17例作为EG组,以同期结肠息肉患儿15例为对照组。采用流式细胞术检测2组患儿外周血中Tfh细胞及其功能性分子可诱导性共刺激分子(ICOS)、程序性细胞死亡蛋白1(PD-1)的表达。结果EG组与对照组患儿外周血Tfh细胞频率的中位数(四分位间距)分别为7.3(2.6)%、2.8(1.4)%,2组比较差异有统计学意义(P<0.05)。EG组与对照组患儿Tfh细胞上ICOS、PD-1的表达水平的中位数(四分位间距)分别为1.5(1.3)%、1.8(3.2)%及0.1(0.2)%、0.7(0.6)%,2组比较差异均有统计学意义(均P<0.05)。EG组患儿外周血中Tfh细胞频率与ICOS、PD-1表达量及免疫球蛋白E(IgE)水平均呈正相关(r=0.746、0.893、0.587,均P<0.05)。结论EG患儿外周血Tfh细胞增多,高表达ICOS、PD-1,处于增殖活化状态;EG患儿外周血Tfh细胞数量与IgE水平呈正相关性;Tfh细胞及其相关分子的异常表达在EG的发生机制中可能起促进作用。Objective To investigate the role and clinical significance of T follicular helper(Tfh)cells in the pathogenesis of eosinophilic gastroenteritis(EG)in children.Methods A total of 17 children diagnosed with EG in the Department of Gastroenterology,Children′s Hospital of Nanjing medical University from October 2018 to January 2020 were recruited as EG group.During the same period,15 children diagnosed with colon polyps were included as control group.Flow cytometry was used to detect Tfh cells and their functional molecules,including inducible costimulatory molecules(ICOS)and programmed cell death protein 1(PD-1)in peripheral blood of the 2 groups.Results The median(interquartile range)of Tfh cell frequency in peripheral blood of children with EG group was 7.3(2.6)%,which was significantly higher than that of controls 2.8(1.4)%(P<0.05).There were significant differences in the median(interquartile range)of ICOS[1.5(1.3)%vs.0.1(0.2)%]and PD-1 expressions[1.8(3.2)%vs.0.7(0.6)%]on Tfh cells between children with EG group and control group(all P<0.05).The frequency of Tfh cells in the peripheral blood of children with EG was positively correlated with the expressions of ICOS(r=0.746,P<0.05)and PD-1(r=0.893,P<0.05),and immunoglobulin E(IgE)level(r=0.587,P<0.05).Conclusions The frequency of Tfh cells in peripheral blood of children with EG significantly increases,which are proliferative and overexpressed with ICOS and PD-1.Moreover,the frequency of Tfh cells of EG patients is positively correlated with the level of IgE.The abnormal expression of Tfh cells may play a promoting role in the mechanism of EG.

关 键 词：嗜酸性粒细胞性胃肠炎 滤泡辅助性T淋巴细胞 诱导性共刺激分子 程序性死亡分子1 免疫球蛋白E 流式细胞术 

分 类 号：R725.7[医药卫生—儿科]