不同条件对薏苡黑穗病菌冬孢子萌发的影响  

Effect of Different Conditions on Teliospore Germination of Ustilago Coicis in Job's Tears Smut

作　　者：练启仙[1] 杨光粉 岑怡红[1] 李祥栋 蒋太英 张万芹[1] LIAN Qixian;YANG Guangfen;CEN Yihong;LI Xiangdong;JIANG Taiying;ZHANG Wanqin(Department of Chemical Biology,Xingyi Normal University for Nationalities,Xingyi,Guizhou 562400;Southwest Guizhou Institue of Karst Regional Development,Xingyi,Guizhou 562400;Agricultural Service Center of Qingshui River Town,Xingyi,Guizhou 562400)

机构地区：[1]兴义民族师范学院,贵州兴义562400 [2]贵州黔西南喀斯特区域发展研究院,贵州兴义562400 [3]兴义市清水河镇农业服务中心,贵州兴义562400

出　　处：《兴义民族师范学院学报》2021年第4期112-119,共8页Journal of Minzu Normal University of Xingyi

基　　金：黔西南州科技基础研究计划项目“贵州薏苡黑穗病菌遗传多样性分析”(2017-1-2);兴义民族师范学院博士科研基金项目“贵州薏苡病害调查及重要病害病原菌多样性分析”(18XYBS03)

摘　　要：为明确薏苡黑穗病菌冬孢子在自然环境下的萌发情况,研究了不同温度、碳源、氮源、培养基、pH、光照等条件对冬孢子萌发的影响,并测定了冬葩子萌发的致死温度。结果表明:温度过高或者过低均抑制其萌发,薏苡黑穗病菌冬孢子萌发的适宜温度为25%2,72h时各温度对冬孢子萌发的影响其差异显著;中性和偏酸条件有利于冬孢子萌发,适宜pH为5~7;不同碳源和氮源中,72h时的冬孢子萌发较好的碳源和氮源分别是乳糖和苯丙氨酸,且它们与其它碳源和氮源间差异显著;燕麦和薏苡液体培养基对冬胞子的萌发相对较好;光暗交替和全黑暗利于冬孢子的萌发,紫外光可抑制冬孢子的萌发;薏苡黑穗病菌冬孢子的致死温度为59℃10min。In order to find out the teliospore germination conditions of Ustilago coicis in the field, this article tested the effect of different temperatures, carbon source, nitrogen source, medium, pH, light and lethal temperatures on the teliospore germination. The results showed that teliospore germination was inhibited by extremely high or low temperature, the optimum temperature for the teliospore germination was 25℃. The neural and acid condition was conductive to the germination of teliospore, and the optimal pH was 5-7;Among different carbon and nitrogen source, lactose and phenylalanine were the best carbon and nitrogen source for the germination of teliospore, which showed significant differences with other carbon and nitrogen sources at 72 h. Oat meal and Job’s Tears liquid medium had relatively high germination rates of teliospore.Alternation of light and dark and total darkness were the appropriate conditions for teliospore germination, and ultraviolet light inhibited the germination of teliospores.The lethal temperature of U. coicis teliospore was 59 ℃ for 10 min.

关 键 词：薏苡 黑穗病 冬孢子 萌发条件 

分 类 号：S435.1[农业科学—农业昆虫与害虫防治]