基于高效液相色谱指纹图谱的多叶棘豆指标成分的含量测定  

Content determination of index components in Oxytropis myriophylla based upon high performance liquid chromatography fingerprint

作　　者：刘洋 岳鑫 王晓琴 LIU Yang;YUE Xin;WANG Xiao-qin(School of Pharmacy,Inner Mongolia Medical University,Inner Mongolia Hohhot 010110,China)

机构地区：[1]内蒙古医科大学药学院,内蒙古呼和浩特010110

出　　处：《中国医院药学杂志》2021年第20期2050-2053,共4页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金项目(编号:81760686);2018年中医药公共卫生服务补助专项“全国中药资源普查项目”;国家中医药管理局中医药创新骨干人才项目(2019)。

摘　　要：目的:建立多叶棘豆HPLC指纹图谱及2个指标成分的含量测定方法。方法:采用HPLC法,Symmetry■C_(18)色谱柱(250 mm×4.6 mm,5.0μm);以乙腈(A)-0.1%乙酸水溶液(B)梯度洗脱;柱温30℃;流速为1.0 mL·min^(-1);检测波长为254 nm。结果:建立了10批多叶棘豆药材的HPLC指纹图谱,相似度在0.937~0.973,共标定了18个共有峰;同时测定了24批药材中槲皮素-7-O-α-鼠李糖-3-O-(6″-反式咖啡酰)-β-D-葡萄糖基-(1→2)-β-D-葡萄糖苷和芦丁的含量,分别为0.02~11.75 mg·g^(-1)和0.01~1.26 mg·g^(-1)。结论:建立的指纹图谱结合含量测定的方法可有效、全面地用于多叶棘豆药材的质量评价。OBJECTIVE To establish high performance liquid chromatography(HPLC)fingerprint of Oxytropis myriophylla and content determination method of two index components.METHODS At 30℃ on a Symmetry■C_(18) column(250 mm×4.6 mm,5.0μm),HPLC was eluted with a gradient program using acetonitrile(A)-0.1% acetic acid aqueous solution(B)as a mobile phase.Flow rate was 1.0 mL·min^(-1) and detection wavelength 254 nm.RESULTS The HPLC fingerprints of 10 batches of Oxytropis were established.The similarity was(0.937-0.973)and 18 common peaks were identified.Simultaneously the contents of quercetin-7-O-α-rhamnopyranosyl-3-O-(6″-trans-caffeoyl)-β-D-glucopyranosyl-(1→2)-β-D-glucopyranoside and rutin in 24 batches were(0.02-11.75)and(0.01-1.26)mg·g^(-1) respectively.CONCLUSION The established fingerprint plus content determination method may be effectively and comprehensively utilized for quality evaluations of Oxytropis myriophylla.

关 键 词：多叶棘豆 指纹图谱 含量测定 槲皮素-7-O-α-鼠李糖-3-O-(6″-反式咖啡酰)-β-D-葡萄糖基-(1→2)-β-D-葡萄糖苷 芦丁 

分 类 号：R284.1[医药卫生—中药学]