振动及电针治疗对缺血缺氧性脑病幼鼠运动功能障碍干预机制的比较研究  

Comparative study on the intervention mechanism of vibration and electroacupuncture on motor dysfunction in young rats with hypoxic-ischemic encephalopathy

作　　者：齐腾澈[1] 王春南[1] 李红[1] QI Teng-che;WANG Chun-nan;LI Hong(The Third Department of Neurological Rehabilitation,Shenyang Children′s Hospital,Liaoning Province,Shenyang 110032,China)

机构地区：[1]沈阳市儿童医院神经康复三科,辽宁沈阳110032

出　　处：《中国当代医药》2021年第31期4-8,F0003,F0004,共7页China Modern Medicine

基　　金：辽宁省自然科学基金指导计划项目(20180550667)。

摘　　要：目的从NF-κB调控Bcl-2抗肌细胞凋亡的角度比较全身振动训练和电针治疗对缺血缺氧性脑病(HIE)幼鼠Bederson评分、腓肠肌纤维组织形态及腓肠肌湿重占体重比的影响,研究针对HIE幼鼠运动功能障碍的形成机制以及治疗机制。方法选取120只7日龄健康SD幼鼠,按照随机数字表法分为4组:假手术组不进行造模及任何干预;其余三组幼鼠HIE造模,模型对照组予每日捆绑;电针治疗组予电针治疗;振动治疗组予振动治疗,疗程均为4周。比较建模前、建模2周后及治疗后各组幼鼠的Bederson评分、治疗后各组幼鼠腓肠肌纤维组织形态、NF-κB基因以及Bcl-2 mRNA表达水平。结果建模2周后,模型对照组、电针治疗组与振动治疗组的Bederson评分高于假手术组,差异有统计学意义(P<0.05);治疗后,电针治疗组与振动治疗组的Bederson评分均低于模型对照组,差异有统计学意义(P<0.05);治疗后,电针治疗组与振动治疗组的Bederson评分比较,差异无统计学意义(P>0.05)。治疗后,振动治疗组与电针治疗组的腓肠肌湿重占体重比均高于模型对照组,差异有统计学意义(P<0.05);振动治疗组的腓肠肌湿重占体重比高于电针治疗组,差异有统计学意义(P<0.05)。治疗后,模型对照组的腓肠肌肌纤维萎缩显著,肌纤维退变,振动治疗组及电针治疗组的肌纤维较模型对照组肌纤维排列紧密,肌横纹规则,电针治疗组肌纤维细胞核增多明显。治疗后,模型对照组、振动治疗组及电针治疗组的Bcl-2 mRNA阳性表达和NF-κB基因表达均低于假手术组,差异有统计学意义(P<0.05);振动治疗组与电针治疗组的Bcl-2 mRNA阳性表达和NF-κB基因表达均高于模型对照组,差异有统计学意义(P<0.05);电针治疗组的Bcl-2 mRNA阳性表达和NF-κB基因表达均高于振动治疗组,差异有统计学意义(P<0.05)。结论通过NF-κB基因调控Bcl-2表达降低骨骼肌细胞抗凋亡能力是导致HIE幼鼠骨Objective To compare the effect of whole-body vibration training and electroacupuncture on Bederson score,gastrocnemius muscle fiber morphology and gastrocnemius muscle wet weight ratio in young rats with hypoxic-ischemic encephalopathy(HIE)from the perspective of Bcl-2 anti-myocyte apoptosis regulated by NF-κB,and to study the formation and treatment mechanism of motor dysfunction in young rats with HIE.Methods A total of 1207-day-old healthy SD young rats were divided into 4 groups by random number table method.No modeling and any intervention were done in sham operation group.HIE model was made in the other three groups:the model control group was bound every day;the electroacupuncture treatment group was treated with electroacupuncture;the vibration treatment group was given vibration treatment for 4 weeks.Bederson scores,fibrous morphology,NF-κB gene and Bcl-2 mrna expression levels of gastrocnemius muscle were compared before modeling,2 weeks after modeling and after treatment.Results After 2 weeks of modeling,Bederson scores of model control group,electroacupuncture group and vibration group were higher than those of sham operation group,and the differences were statistically significant(P<0.05).After treatment,the Bederson scores of electroacupuncture treatment group and vibration treatment group were lower than those of model control group,and the differences were statistically significant(P<0.05).After treatment,there was no statistically significant difference in Bederson score between the electroacupuncture treatment group and the vibration treatment group(P>0.05).After treatment,the wet weight of gastrocnemius in vibration treatment group and electroacupuncture treatment group were higher than that in model control group,and the differences were statistically significant(P<0.05).The proportion of wet weight of gastrocnemius muscle in body weight in vibration treatment group was higher than that in electroacupuncture treatment group,and the difference was statistically significant(P<0.05).After trea

关 键 词：缺血缺氧性脑病 电针治疗 振动治疗 NF-κB BCL-2 

分 类 号：R245[医药卫生—针灸推拿学]