藏紫草醇提物的抗炎作用及其机制  

Anti-inflammatory effects and mechanism of Onosma hookeri ethanol extract

作　　者：周子琪[1] 刘益波[1] 郭珊珊 索朗欧珠[1] ZHOU Zi-qi;LIU Yi-bo;GUO Shan-shan;Suolang-ouzhu(Tibet Vocational Technical College,Lhasa 850030,China)

机构地区：[1]西藏职业技术学院,西藏拉萨850030

出　　处：《中成药》2021年第11期3002-3007,共6页Chinese Traditional Patent Medicine

基　　金：西藏自治区自然科学基金项目(XZ2017ZRG-111)。

摘　　要：目的研究藏紫草醇提物对脂多糖诱导的体内外炎症模型的作用及其机制。方法小鼠腹腔注射LPS建立急性腹膜炎模型,检测藏紫草醇提物对炎症因子水平的抑制作用。通过LPS诱导小鼠巨噬细胞RAW 264.7建立细胞炎症模型,检测藏紫草醇提取物对炎症介质和炎症因子的抑制作用,以及细胞中NF-κB通路相关蛋白(p65、p-IκBα、IκBα)的表达。结果藏紫草醇提物能降低小鼠腹腔灌洗液中及巨噬细胞上清液炎症因子的水平,抑制巨噬细胞中炎症介质iNOS和COX-2的表达及LPS诱导活化的NF-κB信号通路的活性。结论藏紫草醇提取物可能通过抑制NF-κB活化改善体内外炎症。AIM To investigate the effect and mechanism of ethanol extract of Onosma hookeri Clarke.var.longiforum Duthie on LPS-induced inflammation models in vivo and in vitro.METHODS Mice injected intraperitoneally with LPS were developed into acute peritonitis models for detection of the effects of O.hookeri ethanol extract on inflammatory factors.The mouse macrophages RAW 264.7 were induced into cell inflammation models by LPS for investigation of effects of O.hookeri ethanol extract on inflammatory mediators and factors,in addition to the determination of expressions of NF-κB pathway-related proteins(p65,p-IκBα,IκBα)of cells.RESULTS O.hookeri ethanol extract reduced the levels of inflammatory factors in mouse peritoneal lavage fluid and macrophage supernatant fluid,and inhibited the expressions of iNOS and COX-2 and activity of LPS-induced NF-κB signaling pathway activation.CONCLUSION O.hookeri ethanol extract may improve the LPS-induced inflammation via inhibiting the activation of NF-κB.

关 键 词：藏紫草醇提物 RAW264.7 炎症 NF-ΚB 

分 类 号：R285.5[医药卫生—中药学]