丁公藤、大果飞蛾藤投料的冯了性风湿跌打药酒快速鉴别  

Rapid identification of Fengliaoxing Fengshi Dieda Wine prepared with Caulis Erycibes or Porana sinensis

作　　者：刘小妹 杨媛媛 胡静 任慧[1] 崔小敏[1] 李宁 曲彤 陶宏迅 陈志永 LIU Xiao-mei;YANG Yuan-yuan;HU Jing;REN Hui;CUI Xiao-min;LI Ning;QU Tong;TAO Hong-xun;CHEN Zhi-yong(Shaanxi Academy of Traditional Chinese Medicine,Xi’an 710003,China;Department of Pharmacy,No.940 Hospital of the Joint Logistics Support Force of the Chinese People’s Liberation Army,Lanzhou 730050,China;Xi’an Institute for Food and Drug Control,Xi’an 710054,China;Institute of Chinese Medical Sciences,University of Macao,Macao 999078,China)

机构地区：[1]陕西省中医药研究院,陕西西安710003 [2]中国人民解放军联勤保障部队第九四○医院药剂科,甘肃兰州730050 [3]西安市食品药品检验所,陕西西安710054 [4]澳门大学中华医药研究院,澳门999078

出　　处：《中成药》2021年第11期3093-3099,共7页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金面上项目(81973419);国家自然科学基金青年项目(81603264);陕西省重点研发计划一般项目(2019SF-302)。

摘　　要：目的快速鉴别丁公藤、大果飞蛾藤投料的冯了性风湿跌打药酒,并探讨成分差异导致的药酒抗风湿药效差异。方法建立冯了性风湿跌打药酒HPLC指纹图谱,结合主成分分析(PCA)、偏最小二乘判别分析(PLS-DA)发掘不同投料药酒的快速鉴别方法。对差异性成分进行网络药理学研究,探讨成分差异导致的抗风湿药效差异。结果9批样品共标定29个共有峰,通过对照品比对指认其中11个峰,采用PCA及PLS-DA得到3种差异性成分和1种专属性成分,实现不同投料药酒的快速鉴别。差异性成分新绿原酸、隐绿原酸和异绿原酸B主要作用于MAPK、TNF、Chemokine、Toll-like信号通路发挥抗风湿作用。结论该方法可实现以丁公藤、大果飞蛾藤投料的冯了性风湿跌打药酒的快速鉴别,提升冯了性风湿跌打药酒质量控制水平。AIM To rapidly identify Fengliaoxing Fengshi Dieda Wine(FLX)prepared with Caulis Erycibes or Porana sinensis Hemsl,and discuss the difference in anti-rheumatic effect caused by discriminatory compounds.METHODS With the established HPLC fingerprint of FLX,principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA)were employed to explore the rapid identification.Network pharmacology was used to explore the difference of anti-rheumatic effect of the discriminatory compounds.RESULTS Twenty-nine common peaks were calibrated for 9 batches of samples,and 11 characteristic peaks were identified by the reference compounds.Three discriminatory compounds and one specific compound were obtained by PCA and PLS-DA analysis for the rapid identification of FLX prepared with different materials.Network pharmacology study revealed that the discriminatory compounds,neochlorogenic acid,cryptochlorogenic acid and isochlorogenic acid B,mainly act on MAPK,TNF,Chemokine,Toll-like pathways to exert anti-rheumatic effect.CONCLUSION The method developed in this study can rapidly identify FLX prepared with Caulis Erycibes or P.sinensis,and improve its quality control.

关 键 词：冯了性风湿跌打药酒 丁公藤 大果飞蛾藤 差异性成分 抗风湿 快速鉴别 

分 类 号：R282.5[医药卫生—中药学]