SIRT3在人胎盘组织的时空性表达及意义  

The spatiotemporal expression and significance of SIRT3 in the human placenta

作　　者：朱盛兰 张慧婷 江一 陈玉婷 韦丽杰 张婧怡 周璇[1] 冯玲[1] Zhu Shenglan;Zhang Huiting;Jiang Yi(Department of Obstetrics and Gynecology,Tongji Hospital,Tongji Medical College,Huazhong University of Science & Technology,Wuhan 430000)

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430000

出　　处：《现代妇产科进展》2021年第12期896-899,904,共5页Progress in Obstetrics and Gynecology

基　　金：国家重点研究发展计划项目(No:2018YFC1002903)。

摘　　要：目的观察SIRT3在人胎盘组织中的时空性表达及其对滋养细胞侵袭迁移功能的影响。方法免疫组化法和免疫印迹法观察SIRT3在早、中、晚孕人胎盘组织中的定位以及表达。采用RNA干扰技术,下调HTR-8/SVneo中SIRT3的表达。通过Matrigel侵袭实验和划痕实验检测SIRT3对滋养细胞侵袭和迁移功能的影响。结果免疫组化结果表明,人妊娠各阶段胎盘组织均有SIRT3表达,主要定位于细胞滋养细胞、合体滋养细胞和间质绒毛外滋养细胞的细胞浆。Western blot法结果表明,晚孕期胎盘组织中SIRT3蛋白表达水平显著低于早、中孕期(P<0.05),而早孕期和中孕期胎盘组织间SIRT3蛋白表达水平无明显差异(P>0.05)。与阴性对照相比,下调SIRT3表达明显抑制HTR-8/SVneo细胞的迁移和侵袭能力。结论SIRT3可能在胎盘发育过程中发挥重要作用。Objective:To observe the spatiotemporal expression of SIRT3 in the human placenta and its effect on the invasion and migration of trophoblast cells.Methods:Immunohistochemistry and Western blot were used to detect the expression and localization of SIRT3 protein in the placentae at different stages of pregnancy.The effect of SIRT3 knockdown by using small-interfering RNAs(siRNAs)on the phenotype of trophoblast cell line(HTR-8/SVneo)was evaluated.Results:SIRT3 was expressed in human placental tissues at different stages of pregnancy,and was mainly localized within the cytotrophoblasts,syncytiotrophoblast and interstitial extravillous trophoblasts.SIRT3 expression was changed during pregnancy,with the lowest expression level in term placentae.SIRT3 knockdown significantly decreased the migration and invasion of HTR-8/SVneo cells.Conclusions:SIRT3 may play an important role during placental development.

关 键 词：胎盘发育 SIRT3 侵袭 迁移 

分 类 号：R714[医药卫生—妇产科学]