竹叶花椒中总黄酮提取及富集试验研究  

Investigation on Extraction and Enrichment of Flavonoids from Zanthoxylum armatum DC

作　　者：周孟焦 何鑫柱 陈凯[2] 康明[1] 梁晓峰[1,2] ZHOU Meng-jiao;HE Xin-zhu;CHEN Kai;KANG Ming;LIANG Xiao-feng(School of Materials and Engineering,Southwest University of Science and Technology,Mianyang 621010,Sichuan,China;Laboratory of Research Development and Utilization on Traditional Chinese Medicine Resources in Northwest Sichuan,Sichuan College of Traditional Chinese Medicine,Mianyang621010,Sichuan,China)

机构地区：[1]西南科技大学材料科学与工程学院,四川绵阳621010 [2]四川中医药高等专科学校川西北中药材资源研究与开发利用实验室,四川绵阳621010

出　　处：《食品研究与开发》2021年第21期69-73,共5页Food Research and Development

基　　金：四川省中医药管理局重点学科建设项目支持;四川中医药高等专科学校科研项目(19BSZR01)。

摘　　要：采用微波辅助提取法,通过单因素和正交试验优化提取条件,利用聚酰胺-大孔吸附树脂联用法富集纯化提取样品中的总黄酮,研究竹叶花椒中总黄酮的提取和富集纯化工艺。试验确定最佳提取工艺参数为乙醇浓度60%,料液比1∶30(g/mL),微波功率400 W,提取温度80℃,提取时间25 min,总黄酮得率达到9.20%。以60%乙醇为洗脱液,聚酰胺-大孔吸附树脂联合吸附纯化总黄酮收率和纯度分别为43.50%和42.54%,效果优于单独使用吸附剂。To investigate the extraction,enrichment and purification of flavonoids from Zanthoxylum armatum DC,the microwave-assisted extraction method was used to optimize the extraction conditions by single factor and orthogonal test,and the total flavonoids in the samples were enriched and purified by the combined method of polyamide-macroporous adsorption resin.The optimal extraction process was that 60%ethanol,the ration of material to liquid 1∶30(g/mL),microwave power 400 W,extraction temperature 80℃,extraction time 25 min and the total flavonoid extraction rate could reach 9.20%.When the eluent was 60%ethanol,the yield and purity of total flavonoids in the combined adsorption and purification of polyamide-macroporous resin were 43.50%and 42.54%,which was better than that of adsorbent alone.

关 键 词：竹叶花椒 总黄酮 微波辅助法 正交试验 富集纯化 

分 类 号：TQ28[化学工程—有机化工]