环状RNA在妊娠期高血压患者中的表达及潜在作用机制  

Differential expression of circRNA in patients with gestational hypertension and its potential mechanism

作　　者：王健[1] 史云[1] 顾秀峰[1] 李玉明 胡金朋 WANG Jian;SHI Yun;GU Xiufeng;LI Yuming;HU Jinpeng(Department 2 of Cardiology,Tianjin Fourth Central Hospital,Tianjin 300140,China)

机构地区：[1]天津市第四中心医院心内二科,300140 [2]武警特色医学中心研究部,天津300162

出　　处：《淮海医药》2021年第5期461-465,共5页Journal of Huaihai Medicine

基　　金：国家重点研发计划项目(2017YFC1307602);武警后勤部项目(CWJ18L004)。

摘　　要：目的:探索妊娠期高血压患者胎盘组织中环状RNA(circRNA)表达水平及潜在作用机制。方法:选取6名妊娠期高血压患者和6名健康孕产妇作为研究对象,分为妊娠期高血压组(M组)和健康对照组(C组),各组的胎盘组织标本均于胎盘娩出后5 min内获取。采用高通量测序检测circRNA的表达水平,将差异circRNA的宿主基因进行基因本体论(GO)富集分析和京都基因与基因组百科全书(KEGG)通路富集分析。Real-time PCR法检测JAK2和STAT3 mRNA的表达水平。结果:2组样本共有43个差异表达的circRNA,其中下调的circRNA有20个,上调的circRNA有23个,差异倍数最大的3个circRNA分别是circRNA00907、circRNA00427、circRNA00420。GO富集分析显示,主要富集的生物学过程为生物过程的正调控、细胞过程的正调控、多生物过程、生殖过程、白细胞激活;主要富集的细胞组分是胞外区、囊泡、膜的侧面、细胞表面、质膜外侧;主要富集的分子功能是信号受体结合、抗原结合、激素活性、免疫球蛋白受体结合、受体配体活性。靶基因主要富集的信号通路中,仅酪氨酸激酶-信号转导与转录激活子(JAK-STAT)信号通路的富集差异具有统计学意义(P<0.05)。与C组比较,M组患者标本中JAK2和STAT3 mRNA表达水平显著升高,差异均有统计学意义(P<0.05)。结论:与健康对照组比较,circRNA在妊娠期高血压患者胎盘组织中表达存在差异,可能通过调控JAK-STAT信号通路参与妊娠期高血压的进展。Objective:To explore the difference of circRNA expression in placenta of patients with gestational hypertension and its potential mechanism.Methods:Six patients with gestational hypertension and six healthy pregnant women were divided into a gestational hypertension group(group M)and a healthy control group(Group C).Placental tissue samples of each group were obtained within 5 minutes after placenta delivery.High throughput sequencing was used to detect the difference of circRNA expression level,and the host genes of differential circRNA were analyzed by GO enrichment and KEGG pathway enrichment.Results:There were 43 differential circRNA in the two groups,of which 20 were down-regulated and 23 were up-regulated.The largest differences were circRNA00907,circRNA00427 and circRNA00420.GO enrichment analysis showed that the main enrichment biological processes were positive regulation of biological process,positive regulation of cellular process,multi biological process,reproductive process and leukocyte activation;the main enriched cell components were extracellular region,vesicle,side of membrane,cell surface and outside of plasma membrane.The main enriched molecular functions were signal receptor binding,antigen binding,hormone activity,immunoglobulin receptor binding and receptor ligand activity.Among the signal pathways in which the target genes are mainly enriched,only the enrichment difference of JAK-STAT signal pathway is statistically significant(P<0.05).Compared with group C,the expression levels of JAK2 and STAT3 mRNA in group M were significantly higher(P<0.05).Conclusion:Compared with healthy pregnant women,there are differences in the expression of circRNA in placenta of patients with gestational hypertension,which may be involved in the progression of gestational hypertension by regulating JAK-STAT signal pathway.

关 键 词：高血压 妊娠性 环状RNA 胎盘 

分 类 号：R714.246[医药卫生—妇产科学]